Rhythms of Drosophila period gene expression in culture
Rhythms of Drosophila period gene expression in culture
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DOI：
10.1073/pnas.94.8.4092
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发表时间：
1997-04-15
影响因子：
11.1
通讯作者：
Siwicki, KK
中科院分区：
文献类型：
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作者：
Emery, IF;Noveral, JM;Siwicki, KK
果蝇时钟基因period（per）和timeless（tim）已被研究的行为和生物化学，但迄今为止还没有可行的培养系统研究果蝇时钟的细胞生物学。我们培养了附着在中枢神经系统上的蛹环腺，观察了4-7天前胸腺周期基因表达的节律。Per蛋白的每日节律可以被培养物中的光所携带，即使神经活动被河豚毒素阻断。在持续黑暗中保持一周的培养物中，荧光素酶报告基因揭示了生物发光的昼夜节律。作为果蝇第一个昼夜节律培养系统，前胸腺为研究生物钟基因与细胞生理之间的相互作用提供了独特的优势。
The Drosophila clock genes period (per) and timeless (tim) have been studied behaviorally and biochemically, but to date there has been no viable culture system for studying the cell biology of the Drosophila clock. We have cultured pupal ring glands attached to the central nervous system and observed rhythms of period gene expression in the prothoracic gland for 4-7 days. A daily rhythm of Per protein can be entrained by light in culture, even when neural activity is blocked by tetrodotoxin. In cultures maintained for a week in constant darkness, a per-luciferase reporter gene revealed circadian rhythms of bioluminescence. As the first circadian culture system from Drosophila, the prothoracic gland provides unique advantages for investigating the interactions between clock genes and cellular physiology.