Oral administration of bovine lactoferrin accelerates the healing of fracture in ovariectomized rats
Oral administration of bovine lactoferrin accelerates the healing of fracture in ovariectomized rats
复制标题
口服牛乳铁蛋白可加速去势大鼠骨折的愈合
DOI：
10.1007/s00774-020-01105-1
复制
发表时间：
2020-04-29
影响因子：
3.3
通讯作者：
Zhu, Ying
中科院分区：
文献类型：
--
作者：
Li, Wenyang;Hu, Jing;Zhu, Ying
乳铁蛋白具有促进骨生长的作用。然而，乳铁蛋白对脆性骨折愈合过程中的影响尚未研究，因此，本研究的目的是探讨口服乳铁蛋白是否可以促进骨折愈合的OVX动物model.Materials和方法3个月后，双侧卵巢切除术，所有大鼠进行单侧胫骨截骨术，然后随机分为对照组和牛乳铁蛋白（bLF）组。在骨折后4周和8周，动物被处死，骨折的胫骨和血清样品被收集用于evaluation.ResultsOur结果表明，bLF治疗不仅加快了骨生长的早期阶段的OPF愈合，但也缩短了重塑过程中的OPF愈合。与对照组相比，bLF处理诱导骨痂BMD显著增加（4周时增加35.0%，8周时增加39.7%;均p < 0.05），这与骨痂的生物力学强度增强一致，极限力在4周时增加3.39倍（p < 0.05），8周时增加1.95倍（p < 0.05）。此外，bLF给药导致血清BALP水平显著升高，血清TRAP 5 b和TNF-α水平显著降低。结论bLF 85 mg/kg/d口服能有效促进OVX大鼠胫骨骨折愈合，且与对照组相比，bLF治疗组骨痂中RANKL/OPG mRNA比值和TNF-α表达均明显降低。
IntroductionLactoferrin has recently been reported for its potent bone growth effects. However, the effects of lactoferrin on the healing process of fragility fracture have not yet been studied, so the purpose of this study is to investigate whether oral administration of lactoferrin can promote the fracture healing in an OVX animal model.Materials and methodsThree months after bilateral ovariectomy, all rats underwent unilateral tibial osteotomy and were then randomly divided into control group and bovine lactoferrin (bLF) group. At 4 and 8 weeks post-fracture, animals were sacrificed, and the fractured tibiae and serum samples were collected for evaluation.ResultsOur results showed that bLF treatment not only accelerated the bone growth at an early stage of OPF healing but also shortened the remolding process of OPF healing. When compared to control group, bLF treatment induced a significant rise in callus BMD (by 35.0% at 4 weeks and by 39.7% at 8 weeks; both p < 0.05) consistent with enhanced biomechanical strength of the callus, with ultimate force increased by 3.39-fold at 4 weeks (p < 0.05) and 1.95-fold at 8 weeks (p < 0.05). Besides, bLF administration resulted in a substantial increase in serum levels of BALP and a significant decrease in serum levels of TRAP 5b and TNF-α. Moreover, both the RANKL/OPG mRNA ratio and the expression of TNF-α in the callus of bLF-treated group were markedly lower than those in the control group.ConclusionsAt a dose of 85mg/kg/day orally administrated bLF potently promoted the bone healing following tibial fracture in OVX rats.