Endothelial microparticles and platelet and leukocyte activation in patients with the metabolic syndrome
Endothelial microparticles and platelet and leukocyte activation in patients with the metabolic syndrome
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DOI：
10.1016/j.amjcard.2006.01.054
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发表时间：
2006-07-01
影响因子：
2.8
通讯作者：
Ahn, Yeon S.
中科院分区：
文献类型：
--
作者：
Arteaga, Roque B.;Chirinos, Julio A.;Ahn, Yeon S.
越来越多的证据表明代谢综合征（MS）和易患动脉粥样硬化的慢性炎症状态之间存在密切联系。我们研究了33例MS患者和25例健康对照者的白细胞、血小板和内皮活化标志物以及细胞相互作用。用流式细胞术检测血小板中P-选择素的表达，血小板微粒的CD 31表达，内皮微粒的CD 31（EMP 31）、CD 62 E（EMP 62 E）和CD 51（EMP 51）的表达，白细胞与血小板微粒/血小板的结合物和白细胞与内皮微粒的CD 54（EMP 54）的结合物。（5）白细胞CD 11b表达。MS患者的EMP 31水平显著升高，但EMP 62 E水平正常，表明EMP 31水平升高是由于内皮细胞凋亡，而不是激活。EMP 51，EMP 54-淋巴细胞结合物，血小板P-选择素的表达，白细胞中的CD 11b表达，血小板-淋巴细胞结合物也增加了MS患者。血小板-白细胞结合物与白细胞活化相关，表明血小板与白细胞结合调节体内白细胞活化。总之，我们的数据证明了MS患者的内皮细胞微粒释放、血小板和白细胞活化以及EMP和血小板与白细胞的结合增加。（c）2006 Elsevier Inc. All rights reserved.
Accumulating evidence has shown a strong association between the metabolic syndrome (MS) and a chronic inflammatory state predisposing to atherosclerosis. We investigated leukocyte, platelet, and endothelial activation markers and cellular interactions in 33 patients with the MS and 25 healthy controls. Using flow cytometry, we measured: (1) P-selectin expression in platelets; (2) platelet microparticles identified by CD31 expression; (3) endothelial microparticles (EMPs) identified by expression of CD31 (EMP31) CD62E (EMP62E), and CD51 (EMP51); (4) conjugates of leukocytes with platelet microparticles/platelets and with EMPs identified by CD54 (EMP54); and (5) CD11b expression in leukocytes. Patients with the MS had markedly elevated EMP31, although EMP62E levels were normal, suggesting that EMP31 levels were increased because of endothelial cell apoptosis, rather than activation. EMP51, EMP54-lymphocyte conjugates, platelet expression of P-selectin, CD11b expression in leukocytes, and platelet-lymphocyte conjugates were also increased in patients with the MS. Platelet-leukocyte conjugates correlated with leukocyte activation, suggesting that platelet binding to leukocytes regulates leukocyte activation in vivo. In conclusion, our data demonstrate endothelial cell microparticle release, platelet and leukocyte activation, and increased binding of EMPs and platelets to leukocytes in patients with the MS. (c) 2006 Elsevier Inc. All rights reserved.