Controlled release of amoxicillin from hydroxyapatite-coated poly(lactic-co-glycolic acid) microspheres
Controlled release of amoxicillin from hydroxyapatite-coated poly(lactic-co-glycolic acid) microspheres
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DOI：
10.1016/j.jconrel.2008.01.017
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发表时间：
2008-04-21
影响因子：
10.8
通讯作者：
Czemuszka, Jan T.
中科院分区：
文献类型：
--
作者：
Xu, Qingguo;Czemuszka, Jan T.
以十二烷基硫酸钠（SDS）为表面活性剂，采用水包油包固体（s/o/w）法制备了载亲水性抗生素阿莫西林的聚乳酸-羟基乙酸共聚物（PLGA）微球。药物包封率超过40%。采用双恒定组成法在3-6 h内成功地将羟基磷灰石（HA）包覆在带负电荷的PLGA微球表面。HA涂层的PLGA微球（HPLG）的特征在于zeta电位和粒度测量和涂层已被证实是缺钙HA通过分析的X-射线衍射，傅立叶变换红外光谱和波长色散光谱。用扫描电镜研究了HPLG的形貌，用聚焦离子束显微镜对HPLG微球的横截面进行了成像。在PBS（pH 7.4）中的体外药物释放实验显示持续释放曲线至少31天，几乎没有初始突释。它显示了生物可降解聚合物通常观察到的三相药物释放曲线。(c)2008 Elsevier B. V.保留所有权利。
Negatively charged poly(lactic-co-glycolic acid) (PLGA) microspheres with an encapsulated hydrophilic antibiotic (amoxicillin) have been prepared by the solid-in-oil-in-water (s/o/w) method using the anionic surfactant, sodium dodecyl sulfate (SDS). Drug encapsulation efficiency is over 40%. Successful coating of hydroxyapatite (HA) on these negatively charged PLGA microspheres has been achieved by a dual constant composition method in 3-6 h. The HA-coated PLGA microspheres (HPLG) have been characterised by zeta-potential and particle size measurements and the coating has been confirmed to be calcium deficient HA by analysis of X-ray diffraction, Fourier transform infrared spectroscopy and wavelength dispersive spectroscopy. The morphology of HPLG was studied by scanning electron microscopy, and cross sections of HPLG microspheres were prepared and imaged using focused ion beam microscopy. In-vitro drug release experiments in PBS (pH7.4) showed a sustained release profile for at least 31 days with little initial burst release. It shows a triphasic drug release profile commonly observed for biodegradable polymers. (c) 2008 Elsevier B.V. All rights reserved.