Detection of an endophytic fungus of Lolium perenne using enzyme-linked immunosorbent assay (ELISA)
Detection of an endophytic fungus of Lolium perenne using enzyme-linked immunosorbent assay (ELISA)
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DOI：
10.1080/00288233.1984.10430431
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发表时间：
1984-05
影响因子：
1.8
通讯作者：
D. Musgrave
中科院分区：
文献类型：
--
作者：
D. Musgrave
摘要建立了一种检测多年生黑麦草内生真菌的酶联免疫吸附试验（ELISA）方法。据推测是导致黑麦草蹒跚的病原体。ELISA使得能够在低至0.1 μ g/ml的水平下检测牧草植物和种子以及在温室中从受感染的种子生长的植物中的内生菌菌丝体。内生菌的抗体与其他测试的真菌菌丝体的反应性可忽略不计。与传统的光学显微镜检查方法相比，ELISA在常规诊断和实验应用中大大提高了检测的快速性和灵敏度。
Abstract A technique using enzyme-linked immunosorbent assay (ELISA), has been developed for the detection of an endophytic fungus of Lolium perenne L. presumed to be the causative agent of ryegrass staggers. ELISA enabled detection of endophyte mycelium at levels as low as 0.1 ųg/ml, in pasture plants and seed, and in plants grown in the glasshouse from infected seed. Antibodies to the endophyte show negligible reactivity with other fungal mycelia tested. ELISA greatly increased the rapidity and sensitivity of detection in both routine diagnostic and experimental applications, compared with traditional methods oflight microscopy examination.