Diverse function and regulation of CmSnf1 in entomopathogenic fungus Cordyceps militaris
Diverse function and regulation of CmSnf1 in entomopathogenic fungus Cordyceps militaris
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CmSnf1在昆虫病原真菌蛹虫草中的多样性功能及调控
DOI：
10.1016/j.fgb.2020.103415
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发表时间：
2020
影响因子：
3
通讯作者：
Bao Da-Peng
中科院分区：
文献类型：
--
作者：
Wang Ying;Wang Rong;Li Yan;Yang Rui-Heng;Gong Ming;Shang Jun-Jun;Zhang Jin-Song;Mao Wen-Jun;Zou Gen;Bao Da-Peng
SNF1/AMPK蛋白激酶在真菌的发育和分解代谢抑制基因的激活中起重要作用。在这项研究中，我们研究了SNF1同源基因在北虫草(CmSnf1)中的作用。以阿拉伯糖为唯一碳源，CmSnf1缺失突变体的营养生长(Delta CmSnf1)下降了42.2%。最引人注目的是，Delta CmSnf1只产生少量分生孢子，并表现出分生孢子萌发延迟。我们发现CmSnf1是菌丝体穿透昆虫角质层形成子实体所必需的，这与Delta CmSnf1几丁质酶和蛋白酶编码基因的下调是一致的。而虫草素在培养上清液中的含量增加了7倍以上。相应地，虫草素基因簇成员的相对表达水平也有所提高。特别是，与虫草素转移相关的cns4的表达上调了10倍。此外，转录分析表明，CmSnf1调节与细胞自噬和氧化应激耐受相关的基因的表达。我们推测，在环境胁迫下，ATG和SNF1通路可能共同维持不利的环境。我们的研究为探索CmSnf1在北草中的不同功能和调控提供了一个初步的框架，这将为药用真菌分子改良的方向提供更多的线索。
SNF1/AMPK protein kinases play important roles in fungal development and activation of catabolite-repressed genes. In this study, we characterized the role of SNF1 ortholog in Cordyceps militaris (CmSnf1). The vegetative growth of a CmSnf1 deletion mutant was (Delta CmSnf1) reduced by 42.2% with arabinose as a sole carbon source. Most strikingly, the Delta CmSnf1 produced only a few conidia and exhibited delayed conidial germination. We found that CmSnf1 was necessary for mycelium to penetrate the insect cuticle to form the fruiting body on silkworm pupae, consistent with the down-regulation of chitinase- and protease-encoding genes in Delta CmSnf1. However, cordycepin content increased by more than 7 times in culture supernatants. Correspondingly, the relative expression levels of cordycepin gene cluster members were also elevated. In particular, the expression of cns4 associated with cordycepin transfer was up-regulated > 10-fold. Furthermore, transcriptional analysis showed that CmSnf1 regulated the expression of genes involved in cell autophagy and oxidative stress tolerance. We speculated that under environmental stress, both the ATG and SNF1 pathways might collaborate to sustain adverse environments. Our study provides an initial framework to probe the diverse function and regulation of CmSnf1 in C. militaris, which will shed more light on the direction of molecular improvement of medicinal fungi.