Superfamily of genes encoding G protein-coupled receptors in the diamondback moth Plutella xylostella (Lepidoptera: Plutellidae)
Superfamily of genes encoding G protein-coupled receptors in the diamondback moth Plutella xylostella (Lepidoptera: Plutellidae)
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小菜蛾 Plutella xylostella 中编码 G 蛋白偶联受体的基因超家族（鳞翅目：小菜蛾科）
DOI：
10.1111/imb.12171
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发表时间：
2015
影响因子：
2.6
通讯作者：
Shen Jin-Liang
中科院分区：
文献类型：
--
作者：
Wu Shun-Fan;Yu Hua-Yang.;Jiang Tian-Tian;Gao Cong-Fen;Shen Jin-Liang
G蛋白偶联受体(GPCRs)是最大、功能最广泛的细胞膜蛋白超家族，参与调节包括生殖、发育和行为在内的多种生理过程。小菜蛾是世界上分布最广的鳞翅目昆虫之一，也是世界上分布最广的鳞翅目昆虫之一，对几乎所有种类的杀虫剂都产生了抗性。虽然许多昆虫的抗药性机制已经被广泛研究，但对GPCRs INP的研究还很少。小菜蛾属。在本研究中，我们确定了95个可能的GPCR。木星基因组。将所鉴定的GPCR与其在家蚕和果蝇中的同源物进行了比较。我们的结果表明，不同昆虫物种的GPCR可能是通过出生和死亡过程进化而来的。比较昆虫之间的差异之一是短神经肽F受体和脂肪运动激素受体INP的复制。木栓菌B。森喜朗。另一个分歧是耐逆基因mth、inp的数量和多样性的减少。小菜蛾属。出生和死亡过程的进化可能与适应取食行为、繁殖和应激反应有关。小菜蛾属。本研究中发现的一些基因可能是开发新农药的潜在靶点。
G protein‐coupled receptors (GPCRs) are the largest and most versatile superfamily of cell membrane proteins, which mediate various physiological processes including reproduction, development and behaviour. The diamondback moth,Plutella xylostella(Lepidoptera: Plutellidae), is one of the most notorious insect pests, preferentially feeding on cruciferous plants.P. xylostellais not only one of the world's most widespread lepidopteran insects, but has also developed resistance to nearly all classes of insecticides. Although the mechanisms of insecticide resistance have been studied extensively in many insect species, few investigations have been carried out on GPCRs inP. xylostella. In the present study, we identified 95 putative GPCRs in theP. xylostellagenome. The identified GPCRs were compared with their homologues inBombyx moriandDrosophila melanogaster. Our results suggest that GPCRs in different insect species may have evolved by a birth‐and‐death process. One of the differences among compared insects is the duplication of short neuropeptide F receptor and adipokinetic hormone receptors inP. xylostellaandB. mori. Another divergence is the decrease in quantity and diversity of the stress‐tolerance gene,Mth, inP. xylostella. The evolution by the birth‐and‐death process is probably involved in adaptation to the feeding behaviour, reproduction and stress responses ofP. xylostella. Some of the genes identified in the present study could be potential targets for the development of novel pesticides.