First mitochondrial genome of a lugworm (Annelida: Arenicolidae) and its phylogenetic position
First mitochondrial genome of a lugworm (Annelida: Arenicolidae) and its phylogenetic position
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DOI：
10.1017/s0025315422001035
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发表时间：
2023-01-06
影响因子：
1.2
通讯作者：
Nakajima，Nobuyoshi
中科院分区：
文献类型：
--
作者：
Kobayashi，Genki;Itoh，Hajime;Nakajima，Nobuyoshi
环节动物线粒体基因组（有丝分裂基因组）已经被很好地记录下来，基于有丝分裂基因组的系统发育分析为环节动物的进化提供了深刻的启示。然而，一些家族的有丝分裂基因组仍然未知。在此，我们测定了海洋阿巴氏线虫（Abarenicola claparedi oceanica）的完整有丝分裂基因组（15524 bp），这是阿巴氏线虫科的第一个有丝分裂基因组。本种的基因顺序与家蚕各系相同。基于6个不同的数据集进行最大似然系统发育分析，包括43个内群（寡毛纲、水蛭目、蛭目和近缘多毛纲）和2个外群（Siboglinidae），即由蛋白质编码基因（PCGs）和rrna的核苷酸序列以及PCGs的氨基酸序列组成的排列和修剪数据集。基于核苷酸序列的系统发育分析结果比基于氨基酸序列的系统发育分析结果具有更好的支持值。在所有的分析中，沙螨科与马尔丹尼科聚在一起。基于核苷酸序列的分析证实了Terebellidae的单系性，在最近的有丝分裂基因组系统发育研究中，Terebellidae是副系的。我们还基于对PCGs核苷酸序列的ry编码进行了系统发育分析，但得到的系统发育支持值普遍较低。为了更全面地了解系统发育关系，需要对相关群内物种进行额外的有丝分裂基因组测序，这在本研究中是不存在的。
The annelid mitochondrial genomes (mitogenomes) have been well documented, and phylogenetic analyses based on the mitogenomes provide insightful implications for annelid evolution. However, the mitogenomes of some families remain unknown. Herein, we determined the complete mitogenome of the lugworm Abarenicola claparedi oceanica (15,524 bp), representing the first mitogenome from the family Arenicolidae. The gene order of this species is the same as the various lineages in Sedentaria. The maximum likelihood phylogenetic analyses were performed based on six different datasets, including 43 ingroups (oligochaetes, hirudineans, echiurans and closely related polychaetes) and two outgroups (Siboglinidae), namely, aligned and trimmed datasets consisting of the nucleotide sequences of protein-coding genes (PCGs) and rRNAs, and amino acid sequences of PCGs. Phylogenetic analyses based on the nucleotide sequences yielded trees with better support values than those based on the amino acid sequences. Arenicolidae is clustered with Maldanidae in all analyses. Analyses based on nucleotide sequences confirm the monophyly of Terebellidae, which was paraphyletic in recent mitogenomic phylogenetic studies. We also performed the phylogenetic analysis based on the RY-coding of the nucleotide sequences of PCGs only to yield phylogeny with generally low support values. Additional mitogenome sequences of related ingroup species would be needed to comprehensively understand the phylogenetic relationship, which was not present in this study.