Rat 5S rDNA spacer sequences and chromosomal assignment of the genes to the extreme terminal region of chromosome 19.
Rat 5S rDNA spacer sequences and chromosomal assignment of the genes to the extreme terminal region of chromosome 19.
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DOI：
10.1159/000134149
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
H. Suzuki;S. Sakurai;Y. Matsuda
中科院分区：
文献类型：
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作者：
H. Suzuki;S. Sakurai;Y. Matsuda
用5SrRNA编码区两端的特异性引物，通过PCR扩增大鼠5SrRNA基因串联重复的间隔区片段。将两个约1.6kb的扩增片段亚克隆并测序。间隔区序列显示出彼此高度的序列同一性（99.2%），但与类似小鼠克隆的间隔区序列显著不同。小鼠克隆中的同源区域被DNA小片段的复制或缺失中断。在大鼠-5S克隆的-33至-22位，定位了一个12聚体，5 ′-GGCTCTTGGGGC-3 ′，被认为负责有效的转录。以克隆的大鼠5S rDNA片段为探针，通过荧光原位杂交技术对基因进行定位。这些基因仅定位于19号染色体的单个端粒区域。
Fragments of the spacer region of genes for rat 5S ribosomal RNA (rDNA), which are tandemly repeated, were amplified by PCR with primers specific to the two ends of the coding region for 5S rRNA. Two amplified fragments of approximately 1.6 kb were subcloned and sequenced. The spacer sequences showed a high degree of sequence identity to each other (99.2%) but substantial divergence from those of analogous mouse clones. The homologous regions in the mouse clones were interrupted by the duplication or deletion of small segments of DNA. A 12-mer, 5'-GGCTCTTGGGGC-3', thought to be responsible for efficient transcription, was located from position -33 to position -22 in the rat -5S clones. The genes were mapped by fluorescence in situ hybridization with cloned fragments of rat 5S rDNA as probe. The genes were localized exclusively in a single telomeric region of chromosome 19.