Mapping and characterization of a 'speciation gene' in Drosophila.
Mapping and characterization of a 'speciation gene' in Drosophila.
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果蝇“物种形成基因”的定位和表征。
DOI：
10.1017/s0016672300030275
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Orr,HA
中科院分区：
文献类型：
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作者：
Orr,HA
对于造成物种间生殖隔离的基因的身份几乎一无所知。作为“物种形成基因”分子分离的第一步，我绘制了果蝇杂交雄性不育基因的图谱，并对其进行了部分表征。结果表明，D.黑腹果蝇雄性携带D. simulans完全是由于D. simulans染色体（仅包括约5条唾液腺带或约250 kb的DNA）。因此，D.几乎可以肯定的是，拟人的第四条染色体是由一个具有很大作用的单基因（这里称为hms，杂种雄性不育）造成的。Hms是合子作用的，而D. hms的simulans等位基因是完全隐性的。此外，互补试验表明，hms不是D中任何已知位点的等位基因。黑腹菌
Almost nothing is known about the identity of the genes causing reproductive isolation between species. As a first step towards molecular isolation of a ‘speciation gene’, I mapped and partly characterized a gene causing hybrid male sterility in Drosophila. This analysis shows that sterility of D. melanogaster males who carry the ‘dot’ fourth chromosome from D. simulans is due entirely to a very small region of the D. simulans chromosome (including only about 5 salivary gland bands or approximately 250 kb of DNA). Thus the hybrid sterility effect of the D. simulans fourth chromosome is almost surely due to a single gene of very large effect (here named hms, hybrid male sterile). Hms is zygotically acting, and the D. simulans allele of hms is completely recessive. Furthermore, complementation tests suggest that hms is not an allele of any known locus in D. melanogaster.