Sphaeropsidins B and C, phytotoxic pimarane diterpenes from Sphaeropsis sapinea f. sp. Cupressi and Diplodia mutila
Sphaeropsidins B and C, phytotoxic pimarane diterpenes from Sphaeropsis sapinea f. sp. Cupressi and Diplodia mutila
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DOI：
10.1016/s0031-9422(97)00006-x
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发表时间：
1997-06-01
期刊：
影响因子：
3.8
通讯作者：
Motta, A
中科院分区：
文献类型：
--
作者：
Evidente, A;Sparapano, L;Motta, A
从两种引起意大利柏树溃疡病的植物病原真菌Sphaeropsis sapinea f， sp. cupressi和Diplodia mutila中分离到两种具有植物毒性的海玛烷二萜，命名为sphaeropsidins B和C。这两种植物毒素均产生，而残皮草产生球花苷C和A，后者也被报道为主要的植物毒素。通过化学和光谱学方法鉴定，sphaeropsidin B是首次从柏树中分离到的真菌代谢产物，而sphaeropsidin C是两种真菌共同产生的一种新的三环酸海玛烷二萜。在柏树和栎树的断枝上测定，sphaeropsidins B和C可引起大叶柏的枯死，永柏树的褐变和坏死，桂花的黄变，栎树和回肠的坏死，栎树的褐变和坏死。注射到上述柏树和橡树幼苗的皮层组织后，前者呈深褐色变色，内部组织呈褐变和褐变，后者呈棕黑色斑点或坏死灶。在番茄和燕麦等非寄主植物上，球藻苷B和C会导致插枝坏死，茎部变色或变黄。在抗菌实验中，两种化合物对7种试验真菌的菌丝生长均有抑制作用。1997爱思唯尔科学有限公司
Two phytotoxic pimarane diterpenes, named sphaeropsidins B and C, were isolated from two phytopathogenic fungi causing canker diseases of Italian cypress (Cupressus sempervirens L.): Sphaeropsis sapinea f, sp. cupressi and Diplodia mutila. S. sapinea f sp. cupressi produced both phytotoxins, whereas D. mutila produced sphaeropsidins C and A, the latter also being reported as the main phytotoxin of S. sapinea f. s. cupressi. Characterized by chemical and spectroscopic methods, sphaeropsidin B proved to be a known fungal metabolite for the first time isolated from S. sapinea f. sp. cupressi, while sphaeropsidin C is a new tricyclic acid pimarane diterpene produced by both fungi. Assayed on severed twigs of cypress and oak, sphaeropsidins B and C caused dieback on C. macrocarpa, browning and necrosis on C. sempervirens and yellowing on C. arizonica, necrosis on Quercus cerri and e. ilex, and browning and necrosis on Q, robur. When injected into cortical tissues of cypress and oak seedlings mentioned above, they caused on the former dark brown discolouration, browning of internal tissues and browning, and brown-blackish spots or necrotic lesions on the latter. On non-host plants like tomato and oat, sphaeropsidins B an C caused necrosis on cuttings and brown discolouration or stewing on the stem. In the antimicrobial assay, both compounds showed an inhibitory effect on mycelial growth of seven test fungi. (C) 1997 Elsevier Science Ltd.