Measurement of human activated factor X-antithrombin complex by an enzyme-linked differential-antibody immunosorbent assay.
Measurement of human activated factor X-antithrombin complex by an enzyme-linked differential-antibody immunosorbent assay.
复制标题
通过酶联差异抗体免疫吸附测定法测量人活化因子 X-抗凝血酶复合物。
DOI：
10.1016/0003-2697(84)90401-9
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发表时间：
1984
影响因子：
2.9
通讯作者：
Harpel,PC
中科院分区：
文献类型：
--
作者：
Jesty,J;Morrison,SA;Harpel,PC
建立了检测人抗凝血酶与凝血因子Xa复合体的酶联免疫吸附试验(ELISA)。将兔抗人凝血因子X抗体吸附到酶标板上，加入含有Xa-抗凝血酶复合体的样品。然后加入兔抗人抗凝血酶抗体的F(ab‘)2片段，先前用碱性磷酸酶标记，然后通过对硝基苯磷酸的水解法测定结合的标记抗体。在样品中可检测到的最小络合物水平约为0.1 nm。在动力学条件下，将1μm-Ile-Glu-Gly-Arg-氯甲基酮加入到酶标液中，可跟踪Xa-抗凝血酶复合体的生成，并已用于血浆系统，观察到该分析的灵敏度降低了20倍。这种减少被证明完全是由血浆凝血因子X引起的。该方法被用来跟踪罗素蛇毒的凝血系统激活时在去纤化血浆中产生的Xa-抗凝血酶复合体，并与在相同混合物中用X因子激活的放射肽测定的Xa因子的总生成量进行了比较。
An enzyme-linked immunoabsorbent assay (ELISA) has been developed for the measurement of the complex of human antithrombin and Factor Xa. Rabbit anti-human Factor X antibodies are adsorbed to ELISA plates, and samples containing Xa-antithrombin complex are added. This is followed by the addition of F(ab′)2fragments of rabbit antibodies against human antithrombin, previously labeled with alkaline phosphatase, and subsequent measurement of the bound labeled antibody by hydrolysis of p-nitrophenylphosphate. The minimum level of complex detectable in a sample is ca. 0.1 nm. The assay has been used to follow the generation of Xa-antithrombin complex in kinetic situations by the addition of 1 μm Ile-Glu-Gly-Arg-chloromethylketone to the ELISA sampling buffer, and it has also been used in plasma systems, where a 20-fold reduction in the sensitivity of the assay is observed. This reduction was shown to be entirely caused by the plasma Factor X. The assay has been used to follow generation of the Xa-antithrombin complex in defibrinated plasma upon activation of the clotting system with the Factor X-activating protein of Russell's Viper venom, and has been compared with the total generation of Factor Xa, measured by a radiopeptide assay of Factor X activation in the same mixtures.
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血浆中的 α2-纤溶酶抑制剂和 α2-巨球蛋白-纤溶酶复合物。
DOI：
10.1172/jci110253
发表时间：
1981
期刊：
The Journal of clinical investigation
影响因子：
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作者：
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影响因子：
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DOI：
--
发表时间：
1982
期刊：
The Journal of biological chemistry
影响因子：
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作者：
CastellotJr,JJ;Favreau,LV;Karnovsky,MJ;Rosenberg,RD
通讯作者：
Rosenberg,RD
DOI：
10.1016/s0021-9258(18)34900-7
发表时间：
1982-03
期刊：
The Journal of biological chemistry
影响因子：
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作者：
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D. Tollefsen;D. Majerus;M. Blank
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DOI：
--
发表时间：
1974
期刊：
Biochimica et Biophysica Acta
影响因子：
--
作者：
B. Furie;B. Furie;A. Gottlieb;W. J. Williams
通讯作者：
W. J. Williams