HUMAN FC-GAMMA-RIII - CLONING, EXPRESSION, AND IDENTIFICATION OF THE CHROMOSOMAL LOCUS OF 2 FC-RECEPTORS FOR IGG
HUMAN FC-GAMMA-RIII - CLONING, EXPRESSION, AND IDENTIFICATION OF THE CHROMOSOMAL LOCUS OF 2 FC-RECEPTORS FOR IGG
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DOI：
10.1073/pnas.86.3.1013
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发表时间：
1989-02-01
影响因子：
11.1
通讯作者：
MOORE, KW
中科院分区：
文献类型：
--
作者：
PELTZ, GA;GRUNDY, HO;MOORE, KW
通过瞬时表达-免疫选择方法从人白细胞文库中分离编码IgG Fc部分的人受体（Fc γ R）、Fc γ RIII或CD 16的cDNA克隆。该cDNA（pGP 5）编码一个46 kDa的磷脂酰肌醇连接的细胞表面蛋白，具有CD 16决定簇和对人IgG的亲和力。推导的蛋白质序列与鼠受体Fc γ RII α最同源，与人受体Fc γ RII和Fc ε RI的同源性稍低。该cDNA与人白细胞和克隆的人自然杀伤细胞系中的2.2 β-内酰胺酶mRNA杂交。通过对分选的人染色体进行斑点印迹分析，将Fc γ RIII定位于1号染色体。Fc γ RII和Fc γ RIII探针与通过脉冲场凝胶电泳脉冲分离的人基因组DNA的限制性内切酶的杂交证明了两个基因在200个内切酶的最大距离内的物理连接。结果鉴定了人染色体I上至少两个Fc γ R基因的基因座。
A cDNA clone encoding a human receptor for the Fc portion of IgG (Fc.gamma.R), Fc.gamma.RIII or CD16, was isolated from a human leukocyte library by a transient expression-immunoselection procedure. This cDNA (pGP5) encodes a 46-kDa phosphatidylinositol-linked cell surface protein with CD16 determinants and affnity for human IgG. The deduced protein sequence is most homologous to the murine receptor Fc.gamma.RII.alpha., with slightly less homology to the human receptors Fc.gamma.RII and Fc.epsilon.RI. The cDNA hybridizes to a 2.2 kilobase mRNA in human leukocytes and a cloned human natural killer cell line. Fc.gamma.RIII is mapped to chromosome 1 by spot-blot analysis of sorted human chromosomes. Hybridization of Fc.gamma.RII and Fc.gamma.RIII probes to restriction digests of human genomic DNA separated by pulsed by pulsed-field gel electrophoresis demonstrates physical linkage of the two genes within a maximum distance of 200 kilobases. The results identify a locus for at least two Fc.gamma.R genes on human chromosome I.