Pretreatment with interleukin-6 small interfering RNA can improve the survival rate of polymicrobial cecal ligation and puncture mice by down regulating interleukin-6 production
Pretreatment with interleukin-6 small interfering RNA can improve the survival rate of polymicrobial cecal ligation and puncture mice by down regulating interleukin-6 production
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DOI：
10.1016/j.ejphar.2012.05.007
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发表时间：
2012-08-05
影响因子：
5
通讯作者：
Sohn, Seonghyang
中科院分区：
文献类型：
--
作者：
Anower, A. K. M. Mostafa;Shim, Ju A.;Sohn, Seonghyang
白细胞介素 - 6（IL - 6）被认为是严重脓毒症的早期标志物，与发病率和死亡率的增加相关。因此，我们在盲肠结扎穿孔（CLP）前用IL - 6小干扰RNA（siRNA）对雄性ICR小鼠进行预处理，并观察IL - 6下调后小鼠存活率的变化，以及脓毒症期间Th细胞亚群的作用。此外，在不同时间点处死假手术组和CLP手术组小鼠，以测定脓毒症早期和晚期的血清IL - 6水平。IL - 6 siRNA预处理的脓毒症小鼠在长达10天的时间里存活率显著提高（23.3%），并且在第5天血清IL - 6水平显著降低（209.90 ± 0.50 pg/ml；P（此处P值相关内容不完整）
Interleukin-6 (IL-6) is considered to be an early marker of severe sepsis that is associated with increased morbidity and mortality. Therefore, we pretreated male ICR mice with IL-6 small interfering RNA (siRNA) before cecal ligation and puncture (CLP) and observed the changes in their survival in response to down regulation of IL-6, as well as the role of Th subsets during sepsis. In addition, sham and CLP operated mice were sacrificed at different time points to determine the serum IL-6 levels during early and late sepsis. IL-6 siRNA pretreated septic mice showed markedly extended survival (23.3%) up to 10 days and significantly reduced serum IL-6 levels at day 5 (209.90 +/- 0.50 pg/ml; P