Agarose as crystallisation media for proteins II:: trapping of gel fibres into the crystals

Agarose as crystallisation media for proteins II:: trapping of gel fibres into the crystals
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DOI:
10.1107/s0907444902014609
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发表时间:
2002-10-01
影响因子:
2.2
通讯作者:
García-Ruiz, JM
García-Ruiz, JM
中科院分区:
生物学4区
文献类型:
--
作者:
Gavira, JA;García-Ruiz, JM

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在琼脂糖凝胶中生长的蛋白质晶体所施加的结晶压力不会破坏凝胶网络。然而,当蛋白质晶体在琼脂糖凝胶中生长时,它们会捕获琼脂糖纤维。琼脂糖纤维随机分布在晶体中,这解释了为什么它们不会明显影响晶体的衍射质量。
The crystallisation pressure exerted by protein crystals growing in agarose gel does not disrupt the gel network. However, protein crystals trap agarose fibres when they grow in agarose gels. The fibres of agarose are distributed randomly in the crystals explaining why they do not appreciably affect the diffraction quality of the crystal.