Nano-characterization of Jagged-1-educated dendritic cells
Nano-characterization of Jagged-1-educated dendritic cells
复制标题
Jagged-1 诱导的树突状细胞的纳米表征
DOI：
10.2478/s11535-011-0063-3
复制
发表时间：
2011
影响因子：
--
通讯作者：
Jing Liu
中科院分区：
文献类型：
--
作者：
Feiyue Xing;Jiongkun Wang;Z. Fang;M. Hu;Yu Yu;Guoliang Chen;Jing Liu
Jagged-1诱导的树突状细胞（DCs）具有与脂多糖（LPS）诱导的DCs不同的表型。然而，到目前为止，很少有人知道锯齿状-1受过教育的DC的纳米功能。在本研究中，通过原子力显微镜观察Jagged-1修饰的DC的纳米结构。结果表明，经Jagged-1诱导的DC体积、表面积和宽度以及表面突起、伪足和板状伪足的数量均显著高于GM-CSF诱导的DC，但低于LPS诱导的DC。与GM-CSF诱导的DCs相比，Jagged-1诱导的DCs表面粗糙度明显增加，与LPS诱导的DCs相似，但其膜上的颗粒大小和数量明显少于后者。Notch信号通路的γ-分泌酶抑制剂N-[N-（3，5-二氟苯乙酰基）-1-丙氨酰]-S-苯基甘氨酸丁酯可消除Jagged-1引起的DC纳米结构的变化。与LPS诱导的DC相比，Jagged-1诱导的DC高表达NICD、Deltex-1和Hes-1蛋白，中等水平产生IL-12，但不产生IFN-γ，其表面CD 40分子水平明显降低，提示Jagged-1诱导的DC处于半成熟状态，可能具有不同于LPS诱导的DC的独特功能。
Jagged-1-educated dendritic cells (DCs) are of distinct phenotypes different from Lipopolysaccharide (LPS)-educated DCs. However, to date, little is known as to nano-features of Jagged-1-educated DCs. In this study, nanostructure of Jagged-1-educated DCs was observed through atomic force microscopy. Our results showed that the volume, surface area and width of Jagged-1-educated DCs, and the number of protrusion, pseudopodia and lamellapodia on the surface of Jagged-1-educated DCs were significantly more than those of GM-CSF-induced DCs, but less than those of LPS-educated DCs. Compared with GM-CSF-induced DCs, the roughness on the surface of Jagged-1-educated DCs was greatly increased, similar to LPS-educated DCs, but the particle size and number of them on the membrane were markedly less than the latter. The change of DC nanostructure caused by Jagged-1 was abrogated with N-[N-(3,5-difluorophenacetyl)-l-alanyl]-S-phenylglycinet-butyl ester, a γ-secretase inhibitor of Notch signaling pathway. Compared to LPS-educated DCs, Jagged-1-educated DCs highly expressed NICD, Deltex-1 and Hes-1 proteins, moderately produced IL-12, but not IFN-γ, and the level of CD40 molecules on the surface of them was much lower, suggesting that Jagged-1-educated DCs are in a semi-mature status and may have unique functions distinguished from LPS-educated DCs.
影响因子：
32.4
作者：
F. Ouaaz;J. Arron;Ye Zheng;Yongwon Choi;A. Beg
通讯作者：
F. Ouaaz;J. Arron;Ye Zheng;Yongwon Choi;A. Beg
影响因子：
20.3
作者：
Cheng, Pingyan;Nefedova, Yulia;Gabrilovich, Dmitry I.
通讯作者：
Gabrilovich, Dmitry I.