CLONING AND MOLECULAR CHARACTERIZATION OF GENES WHOSE PRODUCTS ALLOW SALMONELLA-TYPHIMURIUM TO PENETRATE TISSUE-CULTURE CELLS
CLONING AND MOLECULAR CHARACTERIZATION OF GENES WHOSE PRODUCTS ALLOW SALMONELLA-TYPHIMURIUM TO PENETRATE TISSUE-CULTURE CELLS
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DOI：
10.1073/pnas.86.16.6383
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发表时间：
1989-08-01
影响因子：
11.1
通讯作者：
CURTISS, R
中科院分区：
文献类型：
--
作者：
GALAN, JE;CURTISS, R
肠上皮的侵袭被认为是沙门氏菌感染发病机制中的重要一步。利用体外系统，我们分离出了一个基因位点 inv，它赋予鼠伤寒沙门氏菌非侵入性菌株穿透组织培养细胞的能力。携带 inv 突变的高毒力鼠伤寒沙门氏菌菌株无法进入 Henle-407 细胞，但其附着于培养细胞的能力不受影响。当给 BALB/c 小鼠口服给药时，鼠伤寒沙门氏菌 inv 突变体比其野生型亲本菌株具有更高的 50% 致死剂量 (LD50)。相反，当菌株腹膜内施用时，观察到的 LD50 没有差异。此外，当口服给药时，inv突变体表现出在派尔氏集结、小肠壁和脾脏中定殖的能力降低，尽管在腹膜内给药时，与野生型亲本菌株相比，它们在脾脏中定殖的能力没有显示出差异。
Invasion of the intestinal epithelium is thought to be an important step in the pathogenesis of Salmonella infections. Using an in vitro system, we have isolated a genetic locus, inv, that confers to a noninvasive strain of Salmonella typhimurium the ability to penetrate tissue culture cells. Highly virulent S. typhimurium strains carrying inv mutations were defective for entry into Henle-407 cells while remaining unaffected in their ability to attach to cultured cells. When administered perorally to BALB/c mice, inv mutants of S. typhimurium had higher 50% lethal doses (LD50) than their wild-type parent strains. To the contrary, there were no differences in the observed LD50 when strains were administered intraperitoneally. In addition, inv mutants presented decreased ability to colonize the Peyer''s patches, the small intestinal wall, and the spleen when administered perorally, although when administered intraperitoneally, they showed no difference in their ability to colonize the spleen compared to the wild-type parent strain.