Oscillating Couette flow for in vitro cell loading.
Oscillating Couette flow for in vitro cell loading.
复制标题
用于体外细胞加载的振荡库埃特流。
DOI：
10.1016/j.jbiomech.2003.11.004
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发表时间：
2004
影响因子：
2.4
通讯作者：
Yokota,Hiroki
中科院分区：
文献类型：
--
作者：
Nalim,Razi;Pekkan,Kerem;Sun,HuiBin;Yokota,Hiroki
Synovial joints are loaded by weight bearing, stretching, and fluid-driven shear. To simulate in vitro fluid-driven shear, we developed an “oscillating Couette flow mechanical shear loader”. Oscillating Couette flow mimics relative motion of articular surfaces; hence, characterizing flow-induced shear by the loader enhances understanding of mechanotransduction in the joint tissue. Here, the analytical and computational models for an oscillating Couette flow were used to predict time-varying shear distribution on a plate surface, applying numerical simulation to evaluate the effects of finite plate dimension in a 2D flow. Shear stress on the plate was significantly different from that in simpler models (unbounded plates and viscous low-frequency flow). High-stress spots appeared near the leading and trailing edges of a moving plate, and a relatively uniform shear region was restricted to the interior area. Stress prediction in an example experimental geometry is presented, where the frequency and finite width effects are feasibly accounted.
