REV3, A SACCHAROMYCES-CEREVISIAE GENE WHOSE FUNCTION IS REQUIRED FOR INDUCED MUTAGENESIS, IS PREDICTED TO ENCODE A NONESSENTIAL DNA-POLYMERASE
REV3, A SACCHAROMYCES-CEREVISIAE GENE WHOSE FUNCTION IS REQUIRED FOR INDUCED MUTAGENESIS, IS PREDICTED TO ENCODE A NONESSENTIAL DNA-POLYMERASE
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DOI：
10.1128/jb.171.10.5659-5667.1989
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发表时间：
1989-10-01
影响因子：
3.2
通讯作者：
LAWRENCE, CW
中科院分区：
文献类型：
--
作者：
MORRISON, A;CHRISTENSEN, RB;LAWRENCE, CW
我们已经克隆了酿酒酵母的REV 3基因的紫外诱变的Rev 3缺陷的互补。该基因的核苷酸序列编码Mr为172，956的预测蛋白质，该蛋白质在爱泼斯坦-巴尔病毒DNA聚合酶中显示出显著的序列相似性，并且与包括人DNA聚合酶α在内的一类DNA聚合酶的其它成员具有显著的序列相似性。和酵母DNA聚合酶I。REV 3蛋白与后两种酶的序列一致性较低，可能与后两种酶的进化关系更远。携带REV 3完全缺失的单倍体是可行的。我们认为S.酿酒酵母依赖于一个专门的DNA聚合酶，这是不需要其他复制过程。REV 3位于距CDC 60 2.8厘摩的染色体XVI上。
We have cloned the REV3 gene of Saccharomyces cerevisiae by complementation of the rev3 defect in UV-induced mutagenesis. The nucleotide sequence of this gene encodes a predicted protein of Mr 172,956 showing significant sequence similarity in Epstein-Barr virus DNA polymerase and to other members of a class of DNA polymerase including human DNA polymerase .alpha. and yeast DNA polymerase I. REV3 protein shows less sequence identity, and presumably a more distant evolutionary relationship, to the latter two enzymes than they do to each other. Haploids carrying a complete deletion of REV3 are viable. We suggest that induced mutagensis in S. cerevisiae depends on a specialized DNA polymerase that is not required for other replicative processes. REV3 is located 2.8 centimorgans from CDC60, on chromosome XVI.