Blood-retinal barrier breakdown in retinitis pigmentosa: light and electron microscopic immunolocalization.
Blood-retinal barrier breakdown in retinitis pigmentosa: light and electron microscopic immunolocalization.
复制标题
DOI：
--
复制
发表时间：
1995
影响因子：
2
通讯作者：
S. Vinores;M. Küchle;N. Derevjanik;J. Henderer;J. Mahlow;W. Green;P. Campochiaro
中科院分区：
文献类型：
--
作者：
S. Vinores;M. Küchle;N. Derevjanik;J. Henderer;J. Mahlow;W. Green;P. Campochiaro
视网膜色素变性（retinitis pigmentosa，RP）患者黄斑水肿可导致视力下降，但血视网膜屏障（blood-retinal barrier，BRB）破坏导致黄斑水肿的部位和机制尚不清楚。了解相关机制可能有助于设计有效的药物治疗来预防或减少RP黄斑水肿。为了探讨这个问题，白蛋白的免疫组织化学染色进行石蜡切片22正常和29 RP影响的眼睛。对样本视网膜、视神经乳头、睫状体和虹膜不同区域的白蛋白外渗程度进行分级。对另外6只正常眼和9只RP受累眼进行白蛋白电镜免疫细胞化学染色。三分之二的患有RP且无其他眼部疾病的患者的眼睛在后视网膜的内部显示血管外白蛋白。即使在没有黄斑囊样水肿（CME）的情况下，这一点也很明显，但患有CME的眼睛显示出广泛的BRB失败。在某些情况下，即使在没有视网膜色素上皮（RPE）的情况下，白蛋白从脉络膜到视网膜的通道也被阻止。电镜免疫细胞化学显示，白蛋白渗透视网膜血管内皮细胞和RPE细胞，显示RP的退行性变化。
Macular edema can contribute to visual loss in the retinitis pigmentosa (RP), but the sites and mechanism of blood-retinal barrier (BRB) breakdown leading to macular edema are not known. An understanding of the mechanisms involved could lead to the design of effective pharmacologic therapy to prevent or minimize macular edema in RP. To investigate this problem, immunohistochemical staining for albumin was performed on paraffin sections of 22 normal and 29 RP-affected eyes. Specimens were graded for extent of albumin extravasation in different regions of the retina, optic nerve head, ciliary body, and iris. Electron microscopic immunocytochemical staining for albumin was performed on an additional 6 normal and 9 RP-affected eyes. Two-thirds of the eyes from patients with RP and no other ocular disorders demonstrated extravascular albumin in the inner portion of the posterior retina. This was evident even in the absence of cystoid macular edema (CME), but eyes that had CME showed extensive BRB failure. In some cases, passage of albumin from the choroid to the retina was prevented even in the absence of the retinal pigment epithelium (RPE). Electron microscopic immunocytochemistry revealed that albumin permeated retinal vascular endothelial cells and RPE cells that showed degenerative changes in RP.