A new renal mitochondrial carrier, KMCP1, is up-regulated during tubular cell regeneration and induction of antioxidant enzymes
A new renal mitochondrial carrier, KMCP1, is up-regulated during tubular cell regeneration and induction of antioxidant enzymes
复制标题
DOI：
10.1074/jbc.m412136200
复制
发表时间：
2005-06-10
影响因子：
4.8
通讯作者：
Pecqueur, C
中科院分区：
文献类型：
--
作者：
Haguenauer, A;Raimbault, S;Pecqueur, C
线粒体载体家族转运多种代谢物穿过线粒体内膜。我们鉴定并克隆了这个家族的一个新成员，KMCP 1（肾线粒体载体蛋白-1），它与以前鉴定的蛋白质BMCP 1（脑线粒体载体蛋白-1）高度同源。Western印迹和原位实验表明，这种载体主要在近端和远端肾小管的肾皮质内表达。KMCP 1在禁食期间和甘油诱导的肾功能衰竭的再生阶段增加。我们发现，这两种情况都与瞬时增加的超氧化物生成酶的表达，其次是增加线粒体代谢和抗氧化防御。鉴于KMCP 1的表达同时发生这些后者的事件，我们建议，KMCP 1参与的情况下，线粒体代谢增加，特别是当细胞的氧化还原平衡倾向于一个促氧化状态。
The mitochondrial carrier family transports a variety of metabolites across the inner mitochondrial membrane. We identified and cloned a new member of this family, KMCP1 ( kidney mitochondrial carrier protein-1), that is highly homologous to the previously identified protein BMCP1 (brain mitochondrial carrier protein-1). Western blotting and in situ experiments showed that this carrier is expressed predominantly within the kidney cortex in the proximal and distal tubules. KMCP1 was increased during fasting and during the regenerative phase of glycerol-induced renal failure. We show that both situations are associated with transiently increased expression of superoxide-generating enzymes, followed by increased mitochondrial metabolism and antioxidant defenses. Given that KMCP1 expression occurs simultaneously with these latter events, we propose that KMCP1 is involved in situations in which mitochondrial metabolism is increased, in particular when the cellular redox balance tends toward a pro-oxidant status.