Tracing dung-derived carbon in temperate grassland using 13C natural abundance measurements
Tracing dung-derived carbon in temperate grassland using 13C natural abundance measurements
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DOI：
10.1016/s0038-0717(00)00022-5
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发表时间：
2000-09-01
影响因子：
9.7
通讯作者：
Ostle, N
中科院分区：
文献类型：
--
作者：
Bol, R;Amelung, W;Ostle, N
为了了解粪便衍生碳在放牧温带草原碳循环中的作用，我们需要一种追踪粪便衍生碳的程序。将C-4粪便应用于C-3草场的天然C-13示踪技术使我们能够成功地量化土壤环境中牛粪的命运。从饲喂玉米(C-4)或多年生黑麦草(C-3)的肉牛身上收集粪便。C-4粪便(Delta(13)C-15.4 ppm)或C-3粪便(Delta(13)C=-25.7 ppm)在温带(C-3)草地上以圆形斑块形式施放，其土壤整体Delta(13)C值为-27.9%。从土壤1-5、5-10和10-20 cm深度采集三份样品，并在施粪后每隔150天从零张力蒸渗仪(安装在30 cm深度)采集样品。对土壤和蒸渗仪溶液样品(-lt；0.7亩m过滤)进行了Delta(13)C和总C的分析，Dung C在土壤剖面的上部5 cm处很容易检测到，但在该深度以下不能检测到。150天后，只有16.6%的粪碳被回收，12.6%的粪碳被回收到土壤(1-5厘米深)，4.0%的粪碳在渗滤液中被回收(安装在30厘米深)。显然，草原生态系统中只保留了一小部分粪便C。(C)2000爱思唯尔科学有限公司。保留所有权利。
To understand the role of dung-derived carbon in the carbon cycle of grazed temperate grasslands, we need a procedure to trace dung-derived C. The natural C-13 tracer technique of applying C-4 dung to a C-3 grass pasture allowed us to successfully quantify the fate of cattle dung in the soil environment. Dung was collected from beef steers fed on either maize (C-4) or perennial ryegrass (C-3). The C-4 dung (delta(13)C - 15.4 parts per thousand) or C-3 dung (delta(13)C = -25.7 parts per thousand) was applied in circular patches to a temperate (C-3) grassland, with a bulk soil delta(13)C value of -27.9%. Triplicate samples were taken from 1-5, 5-10 and 10-20 cm depth in the soil, and from zero tension lysimeters (installed at 30 cm depth) at time intervals of 150 days following dung application. The soil and lysimeter solution samples (< 0.7 mu m-filtered) were analysed for delta(13)C and total C. Dung C was readily detectable in the upper 5 cm of the soil profile, but not below that depth. After 150 days, only 16.6% of the applied dung-C was accounted for, with 12.6% of dung C being recovered in the soil (1-5 cm depth) and 4.0% in leachate waters collected in lysimeters (installed at 30 cm depth). Apparently, only a minor proportion of dung C is retained in the grassland ecosystem. (C) 2000 Elsevier Science Ltd. All rights reserved.