Light-Induced Vasodilation of Coronary Arteries and Its Possible Clinical Implication
Light-Induced Vasodilation of Coronary Arteries and Its Possible Clinical Implication
复制标题
DOI：
10.1016/j.athoracsur.2011.12.062
复制
发表时间：
2012-04-01
影响因子：
4.6
通讯作者：
Prusa, Alexander M.
中科院分区：
文献类型：
--
作者：
Plass, Christian A.;Loew, Hans G.;Prusa, Alexander M.
背景。低强度激光疗法和发光二极管 (LED) 越来越多地用于光疗。它们的治疗效果至少部分是由光诱导的血管舒张介导的。本研究的目的是确定不同光源对冠状动脉的影响。方法。将猪的左冠状动脉切成 4 毫米的环，并通过半导体非热砷化镓二极管激光器或非相干无热红光源进行照射，两者的能量密度相同，高达 16 J/cm(2)。用9、11-二脱氧-11α、9α-环氧甲烷-前列腺素F-2α预收缩后，评估各自的松弛反应。研究了内皮细胞和细胞内途径的作用。结果。暴露于激光后观察到最大血管舒张为 10 J/cm(2) (56.8% +/- 1.2%)，并在 16 J/cm(2) 时降至 43.9% +/- 2.8% (p < 0.003)。在调整曝光时间以在 LED 组中实现等效能量密度后，血管段分别显示出 52.9% +/- 6.5% 和 47.5% +/- 0.6% 的光松弛。任一光源实现的血管舒张在 10 J/cm(2) (p < 0.574) 和 16 J/cm(2) (p < 0.322) 下具有可比性。此外，给予2-(4-羧基苯基)-4,4,5,5-四甲基咪唑啉-1-氧基-3-氧化物（一氧化氮清除剂）和1H-[1,2,4]恶二唑并[4,3-a]喹喔啉-1-酮（鸟苷环化酶抑制剂）可以抑制血管舒张，但不能与L-硝基精氨酸甲酯或L-硝基精氨酸甲酯一起使用。去内皮化。结论。暴露于任一光源的容器在一定的能量密度下都表现出显着且相当的光弛豫。这种作用是由细胞内一氧化氮依赖性机制介导的。由于 LED 光源尺寸小、结构简单且成本低廉，因此可在常规冠状动脉搭桥手术中使用它们，以通过靶血管血管舒张来简化吻合缝合。 (Ann Thorac Surg 2012;93:1181-7) (C) 2012，胸外科医师协会
Background. Low-level laser therapy and light-emitting diodes (LED) are increasingly used in phototherapy. Their therapeutic effects are at least partly mediated by light-induced vasodilation. The aim of this study was to determine the effect of different light sources on coronary arteries.Methods. Porcine left coronary arteries were cut into 4-mm rings that were irradiated either by a semiconductor nonthermal gallium-arsenide diode laser or a noncoherent athermic red light source both with the same energy density up to 16 J/cm(2). After precontraction with 9, 11-dideoxy-11 alpha, 9 alpha-epoxymethano-prostaglandin F-2 alpha, respective relaxation responses were evaluated. The role of endothelium as well as intracellular pathways was investigated.Results. Maximum vasodilation after exposure to laser was observed at 10 J/cm(2) (56.8% +/- 1.2%) and decreased to 43.9% +/- 2.8% at 16 J/cm(2) (p < 0.003). After adjusting exposure time to achieve equivalent energy densities in the LED group, vessel segments revealed photorelaxation of 52.9% +/- 6.5% and 47.5% +/- 0.6%, respectively. Vasodilations achieved by either light source were comparable at 10 J/cm(2) (p < 0.574) and 16 J/cm(2) (p < 0.322). Furthermore, vasodilation could be inhibited by administration of 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (nitric oxide scavenger) and 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (guanocyclase inhibitor) but not with L-nitro-arginine methyl ester or deendothelialization.Conclusions. Vessels exposed to either light source showed a remarkable as well as comparable photorelaxation at definite energy densities. This effect is mediated by an intracellular nitric oxide dependent mechanism. As LED sources are of small size, simple, and inexpensive build-up, they may be used during routine coronary artery bypass surgery to ease suturing of anastomosis by target vessel vasodilation. (Ann Thorac Surg 2012;93:1181-7) (C) 2012 by The Society of Thoracic Surgeons