Electrochemical Processes in Biological Systems
Electrochemical Processes in Biological Systems
复制标题
生物系统中的电化学过程
DOI：
10.1002/9781118899076.ch5
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Butt J
中科院分区：
文献类型：
--
作者：
Butt J
氧化还原酶在自然界中无处不在。它们在呼吸过程中的重要性是公认的，它们在全球范围内对细胞毒素的去除和铁、氮、碳和硫的元素循环都做出了同样重要的贡献。因此，蛋白质膜电化学（PFE）提供了一个强大的工具来解析酶的活性，以告知细胞功能的描述及其在生物技术中的应用。PFE为解析酶活性提供了一个强大的工具，为描述细胞功能及其在生物技术中的应用提供了信息。当开始研究一种新酶时，循环伏安法是评估蛋白质暴露电极的电化学活性的最有效手段。PFE的实验装置简单而通用。再加上只需要极少量的酶，这就可以在许多条件下快速检测酶的活性。
Redox enzymes are ubiquitous in nature. Their importance in respiratory processes is well recognized, and they make equally significant contributions to both the removal of cytotoxins and the elemental cycling of Fe, N, C, and S on the global scale. Consequently, the protein film electrochemistry (PFE) provides a powerful tool for resolving enzyme activity to inform descriptions of cellular function and their application in biotechnology. The PFE provides a powerful tool for resolving enzyme activity to inform descriptions of cellular function and their application in biotechnology. When initiating studies of a new enzyme, cyclic voltammetry to be the most effective means to assess the electrochemical activity of a protein‐exposed electrode. The experimental setup for PFE is simple and versatile. Combined with a requirement for only miniscule amounts of enzyme, this allows for rapid interrogation of enzyme activity in numerous conditions.