Fabrication of water-stable silk fibroin scaffolds through self-assembly of proteins
Fabrication of water-stable silk fibroin scaffolds through self-assembly of proteins
复制标题
通过蛋白质自组装制备水稳定性丝素蛋白支架
DOI：
10.1039/c6ra10670f
复制
发表时间：
2016-01-01
期刊：
影响因子：
3.9
通讯作者：
Fan, Yubo
中科院分区：
文献类型：
--
作者：
Yao, Danyu;Liu, Haifeng;Fan, Yubo
丝素蛋白是一种很有前途的生物材料，在组织工程中有着广泛的应用。然而，目前使用的大多数制造技术需要使用不能彻底去除的有机溶剂。残留的有机溶剂降低了细胞的增殖能力。在这项研究中，我们开发了水不溶性SF支架通过一个简单的温度诱导的方法，没有添加有机或无机物质。在冷冻过程中，温度被用来调节SF的自组装，这直接导致水稳定的多孔支架。与传统的甲醇处理的支架相比，目前的支架呈现出较少的结晶结构，较低的弹性模量和较快的降解速率。此外，人脐静脉内皮细胞（HUVECs）在支架上表现出更强的粘附能力、更好的铺展形态和更快的增殖速率。对支架形成机理的研究表明，目前支架的性能主要受储存时间和温度的影响，储存时间和温度通过控制分子活性来调节SF的自组装过程，导致结构的低结晶度和水不溶性。结果表明，这种低结晶度支架作为软组织修复材料的候选者具有很大的希望。
Silk fibroin (SF) is a promising biomaterial and has been widely used in tissue engineering. However, most of the currently used fabrication techniques require the use of organic solvents that cannot be removed thoroughly. The residual organic solvents reduce the proliferation capacity of cells. In this study, we developed water-insoluble SF scaffolds by a simple temperature induced method without the addition of organic or inorganic substances. Temperature was used to tune the self-assembly of SF during the freezing process, which resulted in water stable porous scaffolds directly. Compared with the traditional methanol treated scaffolds, the current scaffolds presented a less crystalline structure, lower elasticity modulus and faster degradation rate. In addition, human umbilical vein endothelial cells (HUVECs) showed an enhanced adhesion capacity, better spreading morphology and a faster proliferation rate on the scaffold. Investigation of the scaffold formation mechanism revealed that the performance of the current scaffold was dominated by the storage time and temperature, which tuned the self-assembly process of SF by controlling the molecular activity and contributed to the low crystallinity of the structure and water-insolubility. The results indicated that this low crystallinity scaffold holds great promise as a candidate for soft tissue repair materials.