Chromosome Duplication in Saccharomyces cerevisiae.
Chromosome Duplication in Saccharomyces cerevisiae.
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DOI：
10.1534/genetics.115.186452
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发表时间：
2016-07
期刊：
影响因子：
3.3
通讯作者：
Labib K
中科院分区：
文献类型：
--
作者：
Bell SP;Labib K
基因组DNA的准确和完整复制对所有生命都至关重要。在真核细胞中，进行复制的多酶复制体的组装被分成在细胞周期的不同阶段发生的阶段。复制性DNA解旋酶仅在G1期装载在DNA复制起点周围。然后，加载的解旋酶在S期被激活，并与复制性DNA聚合酶和其他辅助蛋白结合。所产生的复制体的功能由检查点蛋白监测，所述检查点蛋白在复制被抑制时保护被捕的复制体并抑制新的起始。复制体还协调核小体的拆卸、装配和姐妹染色单体凝聚的建立。最后，当两个复制体聚合时，它们被分解。对酿酒酵母的研究引领了我们对这些过程的理解。在这里，我们回顾了我们越来越多的分子理解这些事件及其调控。
The accurate and complete replication of genomic DNA is essential for all life. In eukaryotic cells, the assembly of the multi-enzyme replisomes that perform replication is divided into stages that occur at distinct phases of the cell cycle. Replicative DNA helicases are loaded around origins of DNA replication exclusively during G1 phase. The loaded helicases are then activated during S phase and associate with the replicative DNA polymerases and other accessory proteins. The function of the resulting replisomes is monitored by checkpoint proteins that protect arrested replisomes and inhibit new initiation when replication is inhibited. The replisome also coordinates nucleosome disassembly, assembly, and the establishment of sister chromatid cohesion. Finally, when two replisomes converge they are disassembled. Studies in Saccharomyces cerevisiae have led the way in our understanding of these processes. Here, we review our increasingly molecular understanding of these events and their regulation.