NUCLEOPORIN85 is required for calcium spiking, fungal and bacterial symbioses, and seed production in Lotus japonicus
NUCLEOPORIN85 is required for calcium spiking, fungal and bacterial symbioses, and seed production in Lotus japonicus
复制标题
DOI：
10.1105/tpc.106.046938
复制
发表时间：
2007-02-01
期刊：
影响因子：
11.6
通讯作者：
Kawaguchi, Masayoshi
中科院分区：
文献类型：
--
作者：
Saito, Katsuharu;Yoshikawa, Makoto;Kawaguchi, Masayoshi
在百脉根中，迄今为止已经确定了7个遗传位点作为根瘤菌和丛枝菌根真菌共享的共同共生（Sym）途径的组成部分。我们的特点nup 85突变体（nup 85 -1，-2，和-3）所需的两种共生体和克隆相应的基因。当接种球囊霉时，菌丝设法进入表皮细胞之间，但它们不能穿透皮层细胞层。nup 85 -2突变赋予了弱的和温度敏感的共生表型，这导致在22摄氏度下低的丛枝形成，但在18摄氏度下允许植物皮层细胞中显著更高的丛枝形成。另一方面，nup 85突变体不形成结节或形成很少的结节。当用中慢生根瘤菌的结瘤因子处理时，nup 85根表现出高度的根毛分枝，但未能诱导钙尖峰。在未接种条件下生长的幼苗提供硝酸盐，nup 85没有逮捕植物生长，但显着减少种子生产。NUP 85编码与脊椎动物NUP 85具有广泛相似性的推定核孔蛋白。与共生核孔蛋白NUP 133一起，L. NUP 85可能是一个特定的核孔亚复合体的一部分，这是至关重要的真菌和根瘤菌定植和种子生产。
In Lotus japonicus, seven genetic loci have been identified thus far as components of a common symbiosis (Sym) pathway shared by rhizobia and arbuscular mycorrhizal fungi. We characterized the nup85 mutants (nup85-1, -2, and - 3) required for both symbioses and cloned the corresponding gene. When inoculated with Glomus intraradices, the hyphae managed to enter between epidermal cells, but they were unable to penetrate the cortical cell layer. The nup85-2 mutation conferred a weak and temperature-sensitive symbiotic phenotype, which resulted in low arbuscule formation at 22 degrees C but allowed significantly higher arbuscule formation in plant cortical cells at 18 degrees C. On the other hand, the nup85 mutants either did not form nodules or formed few nodules. When treated with Nod factor of Mesorhizobium loti, nup85 roots showed a high degree of root hair branching but failed to induce calcium spiking. In seedlings grown under uninoculated conditions supplied with nitrate, nup85 did not arrest plant growth but significantly reduced seed production. NUP85 encodes a putative nucleoporin with extensive similarity to vertebrate NUP85. Together with symbiotic nucleoporin NUP133, L. japonicus NUP85 might be part of a specific nuclear pore subcomplex that is crucial for fungal and rhizobial colonization and seed production.