Identification of putative methylotrophic and hydrogenotrophic methanogens within sedimenting material and copepod faecal pellets
Identification of putative methylotrophic and hydrogenotrophic methanogens within sedimenting material and copepod faecal pellets
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DOI：
10.3354/ame01585
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发表时间：
2012-01-01
影响因子：
1.4
通讯作者：
Hatton, Angela D.
中科院分区：
文献类型：
--
作者：
Ditchfield, Arlene K.;Wilson, Samuel T.;Hatton, Angela D.
在本研究中，自然和实验室饲养的桡足类沉积颗粒材料和粪便颗粒进行了检查产甲烷菌的存在。与产甲烷菌属、甲烷杆菌属和甲烷叶菌属密切相关的16S rRNA基因序列。的检测，表明在这些样品中不同的产甲烷代谢（氢营养型和甲基营养型甲烷）的潜力。本研究代表了第一个16S rRNA基因为基础的鉴定产甲烷桡足类动物粪便颗粒和重要的是第一次报告的16S rRNA为基础的发生潜在的氢营养型甲烷从海洋颗粒物质，它以前被认为是甲基营养型甲烷占主导地位的产甲烷。厌氧培养实验，使用沉淀颗粒材料和桡足类动物粪便颗粒表现出积极的甲烷生产，并表明，相关的产甲烷群落是可行的测试条件下。
In the present study, sedimenting particulate material and faecal pellets from natural and laboratory-reared copepods were examined for the presence of methanogenic Archaea. 16S rRNA gene sequences closely related to Methanogenium, Methanobacterium and Methanolobus spp. were detected, indicating the potential for different methanogenic metabolisms (hydrogeno trophic and methylotrophic methanogenesis) in these samples. The present study represents the first 16S rRNA gene-based identification of methanogens in copepod faecal pellets and importantly the first reported 16S rRNA-based occurrence of potentially hydrogenotrophic methano gens from marine particulate material, where it was previously thought that methylotrophic methano gens dominated methanogenesis. Anaerobic incubation experiments using sedimenting particulate material and copepod faecal pellets demonstrated active methane production and indicated that the associated methanogenic community was viable under the conditions tested.