Genetic effects of dwarfing gene Rht-5 on agronomic traits in common wheat (Triticum aestivum L.) and QTL analysis on its linked traits
Genetic effects of dwarfing gene Rht-5 on agronomic traits in common wheat (Triticum aestivum L.) and QTL analysis on its linked traits
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DOI：
10.1016/j.fcr.2013.10.007
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发表时间：
2014-02-01
影响因子：
5.8
通讯作者：
Hu, Yin-Gang
中科院分区：
文献类型：
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作者：
Daoura, Bachir Goudia;Chen, Liang;Hu, Yin-Gang
为了评价Rht-5矮化基因对普通小麦农艺性状的遗传效应，分别在2011-2012年和2012-2013年冬小麦生长季培育金麦47号（rht-5）和Marfed M（Rht-5）及其两个亲本杂交衍生的F-2群体和32个F-2:3系。 F-2群体形成作图群体，合并其表型和基因型数据以进行QTL作图。选择F-2群体中的矮个子和高个子个体来组成F-2:3品系。 F-2 群体和 F-2:3 品系的方差分析显示，除生物量和谷物产量外，个体或品系之间的所有研究性状均存在高度显着差异。总体而言，在金麦47背景下，矮化基因Rht-5导致株高降低25.84%，抽穗期推迟1 d，可育分蘖数(-1)增加37.39%，而小穗数(-1)和粒数穗数(-1)分别减少8.07%和22.31%。与矮化亲本相比，在矮化品系中可以观察到抽穗期较早、穗较长、小穗穗较多 (-1) 和谷粒穗较多 (-1) 的个体。株高与花序梗长（0.88）、穗长（0.80）、小穗数（-1）（0.63）、穗粒数（0.64）、生物量（0.47）、籽粒产量（0.56）、收获指数（0.37）呈极显着正相关，与可育分蘖数（-1）（-0.64）呈极显着负相关。根据 F-2 群体中的基因型，用三个多态性标记构建了部分染色体连锁图。采用复合区间作图（CIM）分析，检测到株高、抽穗期、花梗长度和穗粒数（-1）4个QTL，分别占表型变异的11.82%、8.02%、10.32%和15.65%。目前的研究结果有助于在育种计划中正确使用Rht-5矮化基因，以提高小麦的抗倒伏性、产量潜力并提高农艺性状标记辅助选择的效率。 (C) 2013 Elsevier B.V. 保留所有权利。
In order to evaluate the genetic effects of Rht-5 dwarfing gene on agronomic traits in common wheat, F-2 population and 32 F-2:3 lines derived from the cross between Jinmai47 (rht-5) and Marfed M (Rht-5) along with the two parents were grown in the winter wheat growing seasons of 2011-2012 and 2012-2013, respectively. The F-2 population formed the mapping population and their phenotypic and genotypic data were merged for QTL mapping. The dwarf and tall individuals in the F-2 population were selected to compose the F-2:3 lines. Analysis of variance in the F-2 population and F-2:3 lines showed highly significant differences among individuals or lines for all of traits studied, except for biomass and grain yield. In general, in the background of Jinmai47, dwarfing gene Rht-5 was associated with a plant height reduction of 25.84%, delaying heading date by 1 day, increasing the number of fertile tillers plant(-1) by 37.39%, while reducing the number of spikelets spike(-1) and number of grains spike(-1) by 8.07% and 22.31%, respectively. Individuals with earlier heading date, longer spikes, more spikelets spike(-1), and more grains spike(-1) could be observed in the dwarf lines when compared to the dwarf parent. Plant height showed highly positive and significant correlation with peduncle length (0.88), spike length (0.80), number of spikelets spike(-1) (0.63), number of grains spike-1 (0.64), biomass (0.47), grain yield (0.56) and harvest index (0.37), whereas it had negative and highly significant correlation with the number of fertile tillers plant(-1) (-0.64). A partial chromosomal linkage map was constructed with three polymorphic markers based on their genotypes in the F-2 population. Using composite interval mapping (CIM) analysis, four QTLs were detected for plant height, heading date, peduncle length and number of grains spike(-1) and accounted for up to 11.82%, 8.02%, 10.32% and 15.65% of the phenotypic variation, respectively. The results of the current study could be useful for proper use of Rht-5 dwarfing gene in breeding programs to improve lodging tolerance, yield potential in wheat and increase efficiency of marker assisted selection for agronomic traits. (C) 2013 Elsevier B.V. All rights reserved.