DNA microarray detection of nitrifying bacterial 16S rRNA in wastewater treatment plant samples
DNA microarray detection of nitrifying bacterial 16S rRNA in wastewater treatment plant samples
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DOI：
10.1016/j.watres.2005.05.044
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发表时间：
2005-09-01
期刊：
影响因子：
12.8
通讯作者：
Stahl, DA
中科院分区：
文献类型：
--
作者：
Kelly, JJ;Siripong, S;Stahl, DA
建立了一种以硝化细菌16 SrRNA为靶点的寡核苷酸探针微阵列，用于污水处理厂样品的检测。使用来自硝化细菌纯培养物的参考rRNA测试微阵列。从工业废水处理设施收集的样品的表征表明，硝化细菌可以直接通过微阵列杂交检测，而不需要PCR扩增。具体地，微阵列检测亚硝化单胞菌属。但未检测到硝化菌。直接检测的特异性和灵敏度进行了评估，使用芯片上的解离分析，并通过两个独立的分析-一个既定的膜杂交格式和末端限制性片段长度多态性指纹（T-RFLP）。后两项分析还显示，硝化螺菌属和硝化杆菌属是处理厂样本中的贡献种群。DNA微阵列在废水处理系统中的应用，在目前的工作中已经证明，应该提供更好的监测能力和处理系统的过程控制，这是容易受到周期性故障。(c)2005爱思唯尔有限公司保留所有权利。
A small scale DNA microarray containing a set of oligonucleotide probes targeting the 16S rRNAs of several groups of nitrifying bacteria was developed for the monitoring of wastewater treatment plant samples. The microarray was tested using reference rRNAs from pure cultures of nitrifying bacteria. Characterization of samples collected from an industrial wastewater treatment facility demonstrated that nitrifying bacteria could be detected directly by microarray hybridization without the need for PCR amplification. Specifically, the microarray detected Nitrosomonas spp. but did not detect Nitrobacter. The specificity and sensitivity of direct detection was evaluated using on-chip dissociation analysis, and by two independent analyses-an established membrane hybridization format and terminal restriction fragment length polymorphism fingerprinting (T-RFLP). The latter two analyses also revealed Nitrospira and Nitrobacter to be contributing populations in the treatment plant samples. The application of DNA microarrays to wastewater treatment systems, which has been demonstrated in the current work, should offer improved monitoring capabilities and process control for treatment systems, which are susceptible to periodic failures. (c) 2005 Elsevier Ltd. All rights reserved.