Chikungunya, Zika, Mayaro, and Equine Encephalitis virus detection in adult Culicinae from South Central Mato Grosso, Brazil, during the rainy season of 2018
Chikungunya, Zika, Mayaro, and Equine Encephalitis virus detection in adult Culicinae from South Central Mato Grosso, Brazil, during the rainy season of 2018
复制标题
DOI：
10.1007/s42770-021-00646-5
复制
发表时间：
2021-11-17
影响因子：
2.2
通讯作者：
Slhessarenko, Renata Dezengrini
中科院分区：
文献类型：
--
作者：
da Silva Neves, Nilvanei Aparecido;Ferreira, Raquel da Silva;Slhessarenko, Renata Dezengrini
在巴西已经报道了几种引起人类疾病的虫媒病毒。在自然界中，虫媒病毒维持涉及脊椎动物和媒介的生命周期，这可能有助于在马托格罗索州（MT）等热带地区定期重新出现公共卫生问题。在这项研究中，我们在2018年雨季期间在蒙大拿州中南部Cuiaba和Varzea Grande的21个观鸟点采样的蚊子尸体池中寻找虫媒病毒。方法对398个蚊库中的6个城市蚊属2873只（57%）雄蚊和2167只（43%）雌蚊进行虫媒病毒RNA提取和RT-PCR检测。在C6/36细胞中对阳性合并液进行病毒分离。结果102/398个样本池中虫媒病毒检测阳性，其中女性样本66/233（29.6%），男性样本36/165（21.8%），主要集中在5月份（31/102）。基孔肯雅病毒（CHIKV）分布在19个点，寨卡病毒（ZIKV）分布在14个点，马亚罗病毒（MAYV）分布在10个点，东部马脑炎病毒（EEEV）分布在3个点。致倦库蚊（39/89的女性和24/99的男性）为CHIKV、ZIKV和MAYV阳性;埃及伊蚊（Stg）池对于CHIKV、ZIKV和EEEV，白纹伊蚊雌性池（8/29）;以及白背锈菌（2/12）和铁背锈菌雌性库（4/16）的CHIKV。结论蚊媒病毒分子检测在不同地理区域和流行区之间存在很大差异，受遗传特征和微生物组对病毒复制的干扰影响。虽然感染的雌性负责在吸血期间传播给脊椎动物，但由垂直途径产生的CHIKV、ZIKV和MAYV的雄性感染可能导致这些虫媒病毒在其天然宿主中的流行间维持。
Introduction Several arboviruses causing human disease have been reported in Brazil. In nature, arboviruses maintain a lifecycle involving vertebrates and vectors, which may contribute for periodical reemergence of those of public health concern in tropical regions, as Mato Grosso State (MT). In this study, we searched for arboviruses in mosquito body pools sampled during the rainy season of 2018 in 21 bird watching points of Cuiaba and Varzea Grande, South Central MT. Methods In total, 2873 (57%) males and 2167 (43%) females belonging to six urban and sylvatic mosquito genera allocated to 398 pools were subjected to RNA extraction and RT-PCR for arboviruses. Positive pools were subjected to virus isolation in C6/36 cells. Results A total of 102/398 pools, 66/233 (29.6%) of females, and 36/165 (21.8%) of males, mostly sampled in May (31/102), were positive for arboviruses. Chikungunya virus (CHIKV) was distributed in 19 points, Zika virus (ZIKV) was found in 14 points, Mayaro virus (MAYV) in 10 points, and East Equine encephalitis virus (EEEV) in three points. Culex quinquefasciatus pools (39/89 of females and 24/99 of males) were positive for CHIKV, ZIKV, and MAYV; Aedes (Stg) aegypti pools (11/46 of females and 12/33 of males) for CHIKV, ZIKV, MAYV, and EEEV; Aedes albopictus female pools (8/29) for CHIKV, ZIKV, and EEEV; and Psorophora albigenu (2/12) and Psorophora ferox female pools (4/16) for CHIKV. Conclusions Arbovirus molecular detection in mosquito populations varies considerable between geographical regions and epidemics, influenced by genetic characteristics and microbiome interference on virus replication. Although infected females are responsible for the transmission to vertebrates during bloodfeeding, male infection by CHIKV, ZIKV, and MAYV resultant from vertical route could lead to interepidemic maintenance of these arboviruses in their natural reservoirs.