Isolation and characterization of the gene HvFAR1 encoding acyl-CoA reductase from the cer-za.227 mutant of barley (Hordeum vulgare) and analysis of the cuticular barrier functions.
Isolation and characterization of the gene HvFAR1 encoding acyl-CoA reductase from the cer-za.227 mutant of barley (Hordeum vulgare) and analysis of the cuticular barrier functions.
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DOI：
10.1111/nph.19063
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发表时间：
2023-06
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影响因子：
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通讯作者：
Y. Müller;Payal Patwari;Tyll Stöcker;V. Zeisler-Diehl;U. Steiner;Chiara Campoli;Lea Grewe;M. Kuczkowska;Maya Marita Dierig;Sarah B. Jose;A. Hetherington;I. Acosta;H. Schoof;L. Schreiber;P. Dörmann
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作者：
Y. Müller;Payal Patwari;Tyll Stöcker;V. Zeisler-Diehl;U. Steiner;Chiara Campoli;Lea Grewe;M. Kuczkowska;Maya Marita Dierig;Sarah B. Jose;A. Hetherington;I. Acosta;H. Schoof;L. Schreiber;P. Dörmann
角质层是覆盖在地上植物器官上的保护层。我们研究了蜡质在大麦角质层屏障建立中的作用。大麦eceriferum突变体cer-za.227和cer-ye.267表现出蜡质负荷减少，但受影响的基因以及蜡质变化对屏障功能的影响仍不清楚。在cer-za.227和cer-ye.267中测量表皮蜡和渗透性。通过批量分离RNA测序分离突变基因座。通过基因组编辑产生新的cer-za等位基因。CER-ZA蛋白在酵母和拟南芥cer 4 -3中表达后进行了表征。Cer-za.227在编码酰基-CoA还原酶（FAR 1）的HORVU 5 Hr 1G 089230中携带突变。cer-ye.267突变位于编码β-酮脂酰辅酶A合酶（KAS 1）的HORVU 4 Hr 1G 063420，与cer-zh.54等位。在cer-ye.267中，表皮内蜡的量强烈减少。cer-za.227的角质层水分流失和渗透性与野生型（WT）相似，但cer-ye.267的角质层水分流失和渗透性有所增加。去除表皮蜡揭示，表皮内，但不是表皮蜡需要调节表皮蒸腾。cer-za.227和cer-ye.267之间表皮内蜡的差异性减少以及表皮蜡的去除表明表皮屏障功能主要取决于表皮内蜡的存在。
The cuticle is a protective layer covering aerial plant organs. We studied the function of waxes for the establishment of the cuticular barrier in barley (Hordeum vulgare). The barley eceriferum mutants cer-za.227 and cer-ye.267 display reduced wax loads, but the genes affected, and the consequences of the wax changes for the barrier function remained unknown. Cuticular waxes and permeabilities were measured in cer-za.227 and cer-ye.267. The mutant loci were isolated by bulked segregant RNA sequencing. New cer-za alleles were generated by genome editing. The CER-ZA protein was characterized after expression in yeast and Arabidopsis cer4-3. Cer-za.227 carries a mutation in HORVU5Hr1G089230 encoding acyl-CoA reductase (FAR1). The cer-ye.267 mutation is located to HORVU4Hr1G063420 encoding β-ketoacyl-CoA synthase (KAS1) and is allelic to cer-zh.54. The amounts of intracuticular waxes were strongly decreased in cer-ye.267. The cuticular water loss and permeability of cer-za.227 were similar to wild-type (WT), but were increased in cer-ye.267. Removal of epicuticular waxes revealed that intracuticular, but not epicuticular waxes are required to regulate cuticular transpiration. The differential decrease in intracuticular waxes between cer-za.227 and cer-ye.267, and the removal of epicuticular waxes indicate that the cuticular barrier function mostly depends on the presence of intracuticular waxes.