Up-regulation of macrophage migration inhibitory factor induced by endotoxin in experimental otitis media with effusion in mice.
Up-regulation of macrophage migration inhibitory factor induced by endotoxin in experimental otitis media with effusion in mice.
复制标题
内毒素诱导的小鼠实验性中耳炎积液中巨噬细胞迁移抑制因子的上调。
DOI：
10.1080/00016480701714228
复制
发表时间：
2008
影响因子：
1.4
通讯作者：
Juhn,StevenK
中科院分区：
文献类型：
--
作者：
Kariya,Shin;Schachern,PatriciaA;Cureoglu,Sebahattin;Tsuprun,Vladimir;Okano,Mitsuhiro;Nishizaki,Kazunori;Juhn,StevenK
结论中耳注射内毒素导致实验性渗出性中耳炎（OME）小鼠模型产生巨噬细胞迁移抑制因子（MIF）。下调MIF可能成为治疗OME的新途径。目的明确MIF在OME中的作用。材料与方法将小鼠分为两组，中耳注射内毒素或磷酸盐缓冲盐水(PBS)。注射后6 h、12 h或1、3、7、14 d处死小鼠，采用酶联免疫吸附法测定中耳积液中MIF、白细胞介素-1β（IL-1β）和肿瘤坏死因子-α（TNF-α）的浓度。结果内毒素组第1天和第3天的MIF浓度显着高于PBS对照组。内毒素组6小时、12小时、1天和3天的IL-1β浓度显着高于对照组。内毒素组第1天和第3天的TNF-α浓度显着高于对照组。内毒素组中MIF浓度与IL-1β、TNF-α浓度呈正相关。
ConclusionInjection of endotoxin into the middle ear causes production of macrophage migration inhibitory factor (MIF) in an experimental mouse model of otitis media with effusion (OME). Down-regulation of MIF may become a new approach for the management of OME.ObjectiveTo determine the role of MIF in OME.Materials and methodsMice were divided into two groups and their middle ears were injected with either endotoxin or phosphate-buffered saline (PBS). Mice were sacrificed at 6 h, 12 h, or 1, 3, 7, or 14 days after injection and concentrations of MIF, interleukin-1 beta (IL-1β) and tumor necrosis factor-alpha (TNF-α) in middle ear effusions were measured by enzyme-linked immunosorbent assay.ResultsConcentrations of MIF in the endotoxin group at 1 day and 3 days were significantly higher than in the PBS control group. Concentrations of IL-1β in the endotoxin group at 6 h, 12 h, 1 day, and 3 days were significantly higher than in controls. Concentrations of TNF-α in the endotoxin group at 1 day and 3 days were significantly higher than in controls. Concentration of MIF in the endotoxin group was positively correlated with that of IL-1β and TNF-α.