Nucleotide sequence of the 3' region of an infectious human T-cell leukemia virus type II genome.
Nucleotide sequence of the 3' region of an infectious human T-cell leukemia virus type II genome.
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传染性人类 T 细胞白血病病毒 II 型基因组 3 区域的核苷酸序列。
DOI：
10.1073/pnas.81.21.6657
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发表时间：
1984
影响因子：
11.1
通讯作者：
Chen,IS
中科院分区：
文献类型：
--
作者：
Shimotohno,K;Wachsman,W;Takahashi,Y;Golde,DW;Miwa,M;Sugimura,T;Chen,IS
使用可作为感染性转化病毒回收的HTLV-II前病毒克隆测定人T细胞白血病病毒II型（HTLV-II）前病毒DNA 3'区的核酸序列。序列数据表明，在3'区有一个功能未知的区域，大约等于1.6个酶对，类似于以前在人T细胞白血病病毒I型（HTLV-1）中鉴定的X区。在HTLV-Ⅱ的X区存在三个重叠的开放阅读框。这些开放阅读框之一Xc最有可能编码蛋白质产物，因为与其他开放阅读框相比，它与HTLV-1的X-IV区域具有更高的预测氨基酸序列同源性（78%），并且在密码子的第三个核苷酸位置处与X-IV具有更大百分比的碱基差异。包括开放阅读框的X-区序列在HTLV-Ⅱ的两个缺失突变体中是保守的，这与对胎牛血清依赖性降低的Mo细胞亚系有关。
The nucleic acid sequence of the 3' region of human T-cell leukemia virus type II (HTLV-II) proviral DNA was determined using a HTLV-II proviral clone that could be recovered as infectious, transforming virus. The sequence data indicate a region of unknown function of approximately equal to 1.6 kilobase pairs in the 3' region, analogous to the X region previously identified in human T-cell leukemia virus type I (HTLV-I). Three overlapping open reading frames are present in the X region of HTLV-II. One of these open reading frames, Xc, is most likely to encode a protein product, because it has greater predicted amino acid sequence homology (78%) with the X-IV region of HTLV-I and a greater percentage of its base differences with X-IV at the third nucleotide position of codons than do the other open reading frames. Sequences of the X-region that include the open reading frames are conserved in two deletion mutants of HTLV-II, which are associated with a subline of Mo cells with a decreased dependence on fetal bovine serum.