Conserved roles for slit and robo proteins in midline commissural axon guidance
Conserved roles for slit and robo proteins in midline commissural axon guidance
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DOI：
10.1016/s0896-6273(04)00179-5
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发表时间：
2004-04-22
期刊：
影响因子：
16.2
通讯作者：
Tessier-Lavigne, M
中科院分区：
文献类型：
--
作者：
Long, H;Sabatier, C;Tessier-Lavigne, M
在果蝇中，中线处的Slit激活连合轴突上的Robo受体，从而将它们排斥出中线，进入中枢神经系统对侧的不同纵向束。在脊椎动物脊髓中，Robo 1和Robo 2由连合神经元表达，而所有三个Slit同源物在腹侧中线表达。以前对Slit 1、Slit 2双突变脊髓的分析未能揭示连合轴突引导的缺陷。我们在这里报告说，当所有六个狭缝等位基因被删除，许多连合轴突未能离开中线，而其他recross it. In此外，Robo 1和Robo 2单突变体显示的指导缺陷，揭示了这两个受体在指导连合轴突的腹侧和侧索内的不同位置的作用。这些结果证明了Slit/Robo信号在脊椎动物中线连合轴突引导中的关键作用。
In Drosophila, Slit at the midline activates Robo receptors on commissural axons, thereby repelling them out of the midline into distinct longitudinal tracts on the contralateral side of the central nervous system. In the vertebrate spinal cord, Robo1 and Robo2 are expressed by commissural neurons, whereas all three Slit homologs are expressed at the ventral midline. Previous analysis of Slit1;Slit2 double mutant spinal cords failed to reveal a defect in commissural axon guidance. We report here that when all six Slit alleles are removed, many commissural axons fail to leave the midline, while others recross it. In addition, Robo1 and Robo2 single mutants show guidance defects that reveal a role for these two receptors in guiding commissural axons to different positions within the ventral and lateral funiculi. These results demonstrate a key role for Slit/Robo signaling in midline commissural axon guidance in vertebrates.