Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins
Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins
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DOI：
10.1105/tpc.016055
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发表时间：
2004-01-01
期刊：
影响因子：
11.6
通讯作者：
Millar, AH
中科院分区：
文献类型：
--
作者：
Heazlewood, JL;Tonti-Filippini, JS;Millar, AH
通过液相色谱-串联质谱仪获得的大型实验蛋白质组揭示了对拟南芥线粒体功能的新见解。在416个确定的蛋白质中，发现了相当数量的低丰度蛋白质，涉及DNA合成、转录调控、蛋白质复合体组装和细胞信号转导。近20%的实验确定的蛋白质具有未知的功能，这表明植物中有大量未被发现的线粒体功能。通过靶向预测程序，只有大约一半的实验集被预测为线粒体，从而可以评估这些程序在确定植物线粒体蛋白质组方面的优势和局限性。酵母、人类和拟南芥线粒体蛋白质组和普氏立克次体蛋白质组之间可能的同源网络图谱为植物线粒体蛋白质组与其他真核生物的差异提供了详细的见解。这些显示了线粒体核心代谢功能中一组明确的跨物种同源基因，而在许多信号和调节功能上存在相当大的多样性。
A novel insight into Arabidopsis mitochondrial function was revealed from a large experimental proteome derived by liquid chromatography-tandem mass spectrometry. Within the experimental set of 416 identified proteins, a significant number of low-abundance proteins involved in DNA synthesis, transcriptional regulation, protein complex assembly, and cellular signaling were discovered. Nearly 20% of the experimentally identified proteins are of unknown function, suggesting a wealth of undiscovered mitochondrial functions in plants. Only approximately half of the experimental set is predicted to be mitochondrial by targeting prediction programs, allowing an assessment of the benefits and limitations of these programs in determining plant mitochondrial proteomes. Maps of putative orthology networks between yeast, human, and Arabidopsis mitochondrial proteomes and the Rickettsia prowazekii proteome provide detailed insights into the divergence of the plant mitochondrial proteome from those of other eukaryotes. These show a clear set of putative cross-species orthologs in the core metabolic functions of mitochondria, whereas considerable diversity exists in many signaling and regulatory functions.