Substrate dependent differences in morphology and elasticity of living osteoblasts investigated by atomic force microscopy
Substrate dependent differences in morphology and elasticity of living osteoblasts investigated by atomic force microscopy
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DOI：
10.1016/s0927-7765(00)00145-4
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发表时间：
2000-12-30
影响因子：
5.8
通讯作者：
Radmacher, M
中科院分区：
文献类型：
--
作者：
Domke, J;Dannöhl, S;Radmacher, M
我们使用原子力显微镜(AFM)作为测试植入材料生物相容性的工具，通过研究成骨细胞的体外黏附行为来测试植入材料的生物相容性。这项技术使得在亚微米尺度上研究活细胞的细胞形态和细胞力学性质成为可能。细胞在钴铬(CoCr)、钛(Ti)和钛钒(TIV)基质上的粘附性是种植体研究领域的热点问题。将金属基质上活的成骨细胞与玻璃和组织培养聚苯乙烯(PS)上培养的成骨细胞的弹性性能和形态进行比较。此外，还对底物的表面粗糙度进行了表征，并用原子力显微镜观察了与细胞培养液孵育后蛋白质在底物上的表面覆盖率。(C)2000 Elsevier Science B.V.保留所有权利。
We have used the atomic force microscope (AFM) as a tool for testing the biocompatibility of implant materials by investigating the adhesion behavior of osteoblast cells in vitro. This technique allowed the investigation of cytomorphology and cytomechanical properties of livings cells on a submicrometer scale. Cell adhesion was investigated on Cobalt-Chromium (CoCr), Titanium (Ti) and Titanium-Vanadium (TiV) substrates, which are of great interest in the field of implant research. The elastic properties and the morphology of living osteoblasts on the metallic substrates were compared with those of osteoblasts cultured on glass and tissue culture polystyrene (PS). Furthermore, a characterization of the surface roughness of the substrates was performed and the surface coverage of proteins after incubation with cell culture medium on the substrates was observed with the AFM. (C) 2000 Elsevier Science B.V. All rights reserved.