Transglutaminase-mediated transamidation of serotonin, dopamine and noradrenaline to fibronectin: Evidence for a general mechanism of monoaminylation
Transglutaminase-mediated transamidation of serotonin, dopamine and noradrenaline to fibronectin: Evidence for a general mechanism of monoaminylation
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DOI：
10.1016/j.febslet.2012.07.062
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发表时间：
2012-09-21
期刊：
影响因子：
3.5
通讯作者：
Schloss, Patrick
中科院分区：
文献类型：
--
作者：
Hummerich, Rene;Thumfart, Jorg-Oliver;Schloss, Patrick
一些小的GTP酶的活性受5-羟色胺(5-羟色胺)共价转胺化为酶的谷氨酰胺残基的调节。这个过程是由转谷氨酰胺酶(TGase)介导的，被称为“5-羟色胺基化”。此外，神经蛋白和细胞外基质蛋白(如纤维连接蛋白)的5-羟色胺基化已被证实。在这里，我们发现儿茶酚胺、多巴胺(DA)和去甲肾上腺素(NA)抑制纤维连接蛋白的5-羟色胺基化，而且DA和NA本身可以被TGase选择性地转化为纤维连接蛋白。这三种生物来源的单胺也阻断了TGase介导的另一种单胺--单丹西卡维林转胺化为纤维连接蛋白，这表明了TGase介导的“单氨化”的一般机制。(C)2012年，由Elsevier B.V.代表欧洲生化学会联合会出版。
The activity of some small GTPases is regulated by covalent transamidation of serotonin (5-hydropxytryptamien) to glutamine residues of the enzymes. This process is mediated by transglutaminase (TGase) and is termed "serotonylation". In addition, serotonylation of neural proteins and proteins of the extracellular matrix such as fibronectin has been demonstrated. Here we show that the catecholamines dopamine (DA) and noradrenaline (NA) inhibit serotonylation of fibronectin and that DA and NA themselves can be selectively transamidated into fibronectin by TGase. All three biogenic monoamines also block TGase-mediated transamidation of another monoamine, monodansylacadaverine, into fibronectin, suggesting a general mechanism of TGase-mediated "monoaminylation". (C) 2012 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.