Day-night rhythm of acetylcholine in the rat pineal gland
Day-night rhythm of acetylcholine in the rat pineal gland
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大鼠松果体乙酰胆碱的昼夜节律
DOI：
10.1016/s0304-3940(97)00165-1
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发表时间：
1997
影响因子：
2.5
通讯作者：
L. Vollrath
中科院分区：
文献类型：
--
作者：
I. Wessler;T. Reinheimer;F. Bittinger;C. Kirkpatrick;J. Schenda;L. Vollrath
采用高效液相色谱法（HPLC）、胆碱乙酰转移酶（ChAT）酶法和抗ChAT免疫组织化学方法，研究了雄性大鼠松果体胆碱能系统的表达。在白天期间（1200小时）采购的腺体含有大量的乙酰胆碱（0.5 pmol/腺体）。一个类似的内容被发现在松果体48小时的培养期后，即当松果体内的神经纤维已经退化。这有力地表明松果体细胞是含有乙酰胆碱的细胞。为了证实这一结论，我们证明大量的ChAT样免疫反应性松果体细胞。在匀浆腺体中测得的ChAT酶活性（白天）为7±3 nmol/mg/h。乙酰胆碱含量以及ChAT酶活性增加约10倍，松果体在夜间（2400小时）。本研究首次证明了大鼠松果体细胞中ChAT和乙酰胆碱的昼夜节律的存在。松果体乙酰胆碱的功能尚不清楚，但有迹象表明，乙酰胆碱可能抑制松果体内交感神经纤维释放去甲肾上腺素，从而抑制褪黑激素的合成。
Using high-performance-liquid-chromatography (HPLC) measurement of acetylcholine, choline acetyltransferase (ChAT) enzyme assay and anti-ChAT immunohistochemistry, we have investigated the expression of the cholinergic system in pineal glands of male rats. Glands procured during the day period (1200 h) contained significant amounts of acetylcholine (0.5 pmol/gland). A similar content was found in pineal glands after a 48 h culture period, i.e. when the intrapineal nerve fibres have degenerated. This strongly indicates that the pinealocytes are the cells which contain acetylcholine. To confirm this conclusion we demonstrate substantial ChAT-like immunoreactivity in pinealocytes. ChAT enzyme activity measured in homogenized glands (day period) was 7±3 nmol/mg per h. Acetylcholine content as well as ChAT enzyme activity increased about 10-fold in pineal glands during the night period (2400 h). The present study demonstrates for the first time the presence of a day-night rhythm of ChAT and acetylcholine in rat pinealocytes. The function of pineal acetylcholine is not clear, but there are indications that acetylcholine may depress noradrenaline release from intrapineal sympathetic fibres and hence melatonin synthesis.
牛松果体毒蕈碱胆碱能受体位点特性的研究。
DOI：
10.1016/0742-8413(89)90160-6
发表时间：
1989
期刊：
Comparative biochemistry and physiology. C, Comparative pharmacology and toxicology
影响因子：
--
作者：
Govitrapong,P;Phansuwan-Pujito,P;Ebadi,M
通讯作者：
Ebadi,M