EVIDENCE THAT DIFFERENT INTEGUMENTAL PHOSPHATASES EXIST DURING DEVELOPMENT IN THE MEDITERRANEAN FRUIT-FLY CERATITIS-CAPITATA - POSSIBLE INVOLVEMENT IN PUPARIATION
EVIDENCE THAT DIFFERENT INTEGUMENTAL PHOSPHATASES EXIST DURING DEVELOPMENT IN THE MEDITERRANEAN FRUIT-FLY CERATITIS-CAPITATA - POSSIBLE INVOLVEMENT IN PUPARIATION
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DOI：
10.1016/0305-0491(91)90198-m
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发表时间：
1991-01-01
影响因子：
2.2
通讯作者：
MARMARAS, VJ
中科院分区：
文献类型：
--
作者：
BOURTZIS, K;PSACHOULIA, C;MARMARAS, VJ
1.“深色”突变体和“野生”Ceratitis headata 菌株中的外皮磷酸酶在化蛹期间仅水解磷酸酪氨酸和β-甘油磷酸，而在“白色”突变体中，它优先水解所有测试的底物，并优先选择 ATP 和 AMP。2。 在蛹中，对于所有测试的三种品系，最佳底物是 ATP 和 AMP.3。 电泳和动力学分析表明，幼虫和成虫的体被磷酸酶同工酶是不同的酶实体。 4． 在幼虫向蛹转变过程中，其他三种双翅目动物和一种鳞翅目动物的珠被也显示出磷酸酶活性，并且发现最佳底物也是磷酸酪氨酸。
1. The integumental phosphatase in "dark" mutant and "wild" Ceratitis capitata strains hydrolyses only phosphotyrosine and beta-glycerophosphate during pupariation while in "white" mutant it hydrolyses all the substrates tested with a preference toward ATP and AMP.2. In the pupal, for all three strains tested, the best substrates are ATP and AMP.3. Electrophoretic and kinetic analysis suggested that the larval and pharate adult isoenzymes of integumental phosphatase are different enzyme entities.4. Phosphatase activity was also demonstrated in the integuments of three other dipterans and one lepidopteran during the transition of larvae to pupae and the best substrate was revealed to be also phosphotyrosine.