Cellular Cargo Delivery: Toward Assisted Fertilization by Sperm-Carrying Micromotors
Cellular Cargo Delivery: Toward Assisted Fertilization by Sperm-Carrying Micromotors
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DOI：
10.1021/acs.nanolett.5b04221
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发表时间：
2016-01-01
期刊：
影响因子：
10.8
通讯作者：
Schmidt, Oliver G.
中科院分区：
文献类型：
--
作者：
Medina-Sanchez, Mariana;Schwarz, Lukas;Schmidt, Oliver G.
我们提出了一种新型的混合微马达，其中定制的微螺旋作为马达用于运输运动缺陷的精子细胞，以帮助它们执行其自然功能。我们的研究结果表明，金属涂层聚合物微螺旋是适合于这项任务，由于有效的，可控的，无害的三维运动行为。我们设法捕获，运输和释放单个不动的活精子细胞在流体通道，允许模仿生理条件。受精的重要步骤是采用适当的精子选择和卵母细胞培养方法。尽管在用人工机动精子实现成功受精的道路上仍然存在一些挑战，但我们相信，这种新的辅助生殖方法的潜力已经可以通过目前的工作来实现。
We present artificially motorized sperm cells a novel type of hybrid micromotor, where customized microhelices serve as motors for transporting sperm cells with motion deficiencies to help them carry out their natural function. Our results indicate that metal-coated polymer microhelices are suitable for this task due to potent, controllable, and nonharmful 3D motion behavior. We manage to capture, transport, and release single immotile live sperm cells in fluidic channels that allow mimicking physiological conditions. Important steps toward fertilization are addressed by employing proper means of sperm selection and oocyte culturing. Despite the fact that there still remain some challenges on the way to achieve successful fertilization with artificially motorized sperms, we believe that the potential of this novel approach toward assisted reproduction can be already put into perspective with the present work.