Three-dimensional microscopy and sectional image reconstruction using optical scanning holography.
Three-dimensional microscopy and sectional image reconstruction using optical scanning holography.
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DOI：
10.1364/ao.48.00h113
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发表时间：
2009-12
期刊：
影响因子：
1.9
通讯作者：
E. Lam;Xin Zhang;Huy Vo;T. Poon;G. Indebetouw
中科院分区：
文献类型：
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作者：
E. Lam;Xin Zhang;Huy Vo;T. Poon;G. Indebetouw
快速采集和高轴向分辨率是三维显微镜的两个主要要求。然而，它们有时是相互冲突的：成像方式，如共焦成像可以提供上级分辨率的代价是在不同的轴向平面上的顺序采集，这是一个耗时的过程。光学扫描全息术（奥什）有望在这两个目标之间实现良好的权衡。只需一次扫描，我们就可以在数字全息图中捕获整个三维体积;然后可以处理数据以获得各个部分。成像系统的精确建模是设计适当的图像重建算法的关键，特别是对于必须考虑随机噪声和其他成像缺陷的真实的数据。在本文中，我们证明了部分图像重建应用逆成像切片技术的实验奥什数据的生物标本和可视化的部分使用OSA互动科学出版软件。
Fast acquisition and high axial resolution are two primary requirements for three-dimensional microscopy. However, they are sometimes conflicting: imaging modalities such as confocal imaging can deliver superior resolution at the expense of sequential acquisition at different axial planes, which is a time-consuming process. Optical scanning holography (OSH) promises to deliver a good trade-off between these two goals. With just a single scan, we can capture the entire three-dimensional volume in a digital hologram; the data can then be processed to obtain the individual sections. An accurate modeling of the imaging system is key to devising an appropriate image reconstruction algorithm, especially for real data where random noise and other imaging imperfections must be taken into account. In this paper we demonstrate sectional image reconstruction by applying an inverse imaging sectioning technique to experimental OSH data of biological specimens and visualizing the sections using the OSA Interactive Science Publishing software.