Bazooka is a permissive factor for the invasive behavior of discs large tumor cells in Drosophila ovarian follicular epithelia
Bazooka is a permissive factor for the invasive behavior of discs large tumor cells in Drosophila ovarian follicular epithelia
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DOI：
10.1242/dev.00420
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发表时间：
2003-05-01
期刊：
影响因子：
4.6
通讯作者：
Jan, YN
中科院分区：
文献类型：
--
作者：
Abdelilah-Seyfried, S;Cox, DN;Jan, YN
果蝇Bazooka和非典型蛋白激酶C是上皮极性和粘附所必需的。我们在这里表明，野生型火箭筒功能是需要在细胞侵袭上皮滤泡细胞突变体的肿瘤抑制光盘大。克隆研究表明，滤泡细胞Bazooka作为一个允许的因素，在细胞入侵，可能是通过稳定入侵的体细胞和他们的基质，生殖细胞之间的粘附。遗传上位性实验表明，火箭筒作用于下游的光盘大肿瘤细胞的侵袭。相比之下，在边缘细胞的迁移过程中，Bazooka的功能是细胞侵袭和运动，而是需要细胞自主地介导细胞粘附在迁移的边缘细胞簇。综上所述，这些研究揭示了Bazooka在上皮滤泡细胞的不同类型的侵袭行为中具有明显的功能，可能是通过调节滤泡细胞之间或滤泡细胞与其种系基质之间的粘附来实现的。
Drosophila Bazooka and atypical protein kinase C are essential for epithelial polarity and adhesion. We show here that wild-type bazooka function is required during cell invasion of epithelial follicle cells mutant for the tumor suppressor discs large. Clonal studies indicate that follicle cell Bazooka acts as a permissive factor during cell invasion, possibly by stabilizing adhesion between the invading somatic cells and their substratum, the germline cells. Genetic epistasis experiments demonstrate that bazooka acts downstream of discs large in tumor cell invasion. In contrast, during the migration of border cells, Bazooka function is dispensable for cell invasion and motility, but rather is required cell-autonomously in mediating cell adhesion within the migrating border cell cluster. Taken together, these studies reveal Bazooka functions distinctly in different types of invasive behaviors of epithelial follicle cells, potentially by regulating adhesion between follicle cells or between follicle cells and their germline substratum.