Allozyme analysis of cryptic species in the Asplenium nidus complex from West Java, Indonesia
Allozyme analysis of cryptic species in the Asplenium nidus complex from West Java, Indonesia
复制标题
印度尼西亚西爪哇巢铁角复合体中隐秘物种的同种酶分析
DOI：
10.1007/s10265-002-0060-8
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发表时间：
2002
影响因子：
2.8
通讯作者：
N. Murakami
中科院分区：
文献类型：
--
作者：
Y. Yatabe;D. Darnaedi;N. Murakami
在各种蕨类植物中，大量的rbcL序列变异已被报道，这是可能的，这些物种包含几个生殖隔离的隐蔽物种。在我们对铁角蕨的前期研究中，这表明，生长在Mt.印度尼西亚西爪哇省的Halimun国家公园由几个神秘的物种组成，这些物种是基于交叉实验的结果rbcL序列类型。在这项研究中，我们研究了在西爪哇发现的五种bcL序列类型的等位酶多态性，以检验A.根据therbcL序列划分的巢是独立的孟德尔种群，基因流被彼此的生殖隔离所破坏。计算的固定指数表明，每个rbcL型的个体至少在调查地点是随机杂交的。然而，theserbcL为基础的组合基因分化的等位酶，编码在他们的核基因组，它也表明，基因流被破坏，即使在同域分布对的rbcL序列类型。因此，我们的研究结果支持西爪哇的fiverbcL序列类型是潜在的神秘物种的观点。
In various fern species, a large amount ofrbcLsequence variation has been reported, and it is possible that these species contain several reproductively isolated cryptic species. In our previous study onAsplenium nidusL., it was suggested that the plants growing in Mt. Halimun National Park, West Java, Indonesia, consist of several cryptic species based on the results of crossing experiments amongrbcLsequence types. In this study, we examined allozyme polymorphisms of fiverbcLsequence types found in West Java in order to test the hypothesis that the assemblages ofA. nidusdelimited based on therbcLsequences are separate Mendelian populations and gene flow is disrupted by reproductive isolation from one another. The calculated fixation indices suggested that the individuals in eachrbcLtype are randomly crossing at least in the investigated localities. Nevertheless, theserbcL-based assemblages were genetically differentiated in allozymes that are encoded in their nuclear genomes, and it is also suggested that gene flow is disrupted even between sympatrically distributed pairs ofrbcLsequence types. Therefore, our findings support the view that the fiverbcLsequence types in West Java are potential cryptic species.