ALLELIC LOSS AT CHROMOSOME BAND 8P21.3-P22 IS ASSOCIATED WITH PROGRESSION OF HEPATOCELLULAR-CARCINOMA
ALLELIC LOSS AT CHROMOSOME BAND 8P21.3-P22 IS ASSOCIATED WITH PROGRESSION OF HEPATOCELLULAR-CARCINOMA
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DOI：
10.1002/gcc.2870070307
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发表时间：
1993-07-01
影响因子：
3.7
通讯作者：
NAKAMURA, Y
中科院分区：
文献类型：
--
作者：
EMI, M;FUJIWARA, Y;NAKAMURA, Y
人肝细胞癌（HCC）常表现出8号染色体短臂上位点的杂合性丢失。为了确定肝癌中8 p上常见的缺失区域，我们使用20个限制性片段长度多态性标记对142例肝癌进行了详细的缺失定位。在124例有信息的病例中，56例（45%）显示肿瘤中的等位基因丢失。其中一半以上的人在短臂上的每个位点都丢失了等位基因，而另一些人则部分或完全丢失了等位基因。cMSR-32和cC 18 -245侧翼的8-cM区域在8p21.3-p22处的共同缺失表明在该区间存在肿瘤抑制基因。在51例有详细临床资料的病例中，研究了8 p等位基因丢失与临床病理参数之间的相关性;在T1期的5个肿瘤中没有观察到8 p等位基因丢失（0%），在T2期的20个肿瘤中有9个（45%），在T3/T4期的26个肿瘤中有17个（65%）。在低分化肿瘤中观察到8 p等位基因丢失的频率（10/14，71%）高于高分化肿瘤（3/13，23%）。8 p等位基因丢失与晚期临床分期和低分化的相关性表明，8 p上肿瘤抑制基因的丢失或失活可能在HCC的进展中起作用。 (C)1993 Wiley-Liss，Inc.
Human hepatocellular carcinomas (HCC) frequently show loss of heterozygosity at loci on the short arm of chromosome 8. To define a region on 8p commonly deleted in HCC, we used 20 restriction fragment length polymorphism markers to carry out detailed deletion mapping in 142 HCC. Of the 124 informative cases, 56 (45%) showed allelic losses in tumors. While more than half of them had lost alleles at every locus on the short arm, others lost them partially or interstitially. Common deletion of an 8-cM region flanked by cMSR-32 and cC18-245, at 8p21.3-p22, suggested the presence of a tumor suppressor gene(s) in this interval. Correlation between allelic losses on 8p and clinicopathological parameters were examined in the 51 cases for which detailed clinical data were available; allelic losses on 8p were observed in none of five tumors at stage T1 (0%), nine of 20 tumors at stage T2 (45%), and 17 of 26 tumors at stage T3/T4 (65%). A higher frequency of allelic losses on 8p was observed in poorly differentiated tumors (10/14, 71%) than in well differentiated tumors (3/13, 23%). The association of allelic losses on 8p with advanced clinical stage and poor differentiation implies that loss or inactivation of a tumor suppressor gene(s) on 8p may play a role in the progression of HCC. (C) 1993 Wiley-Liss, Inc.