Programming biological operating systems: genome design, assembly and activation
Programming biological operating systems: genome design, assembly and activation
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DOI：
10.1038/nmeth.2894
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发表时间：
2014-05-01
期刊：
影响因子：
48
通讯作者：
Gibson, Daniel G.
中科院分区：
文献类型：
--
作者：
Gibson, Daniel G.
在过去20年里，DNA技术在阅读和写入遗传密码方面得到了发展，当第一个合成细胞在4年前被创造出来时，这种技术就开始融合了。这项工作的成果是一套非凡的工具，用于合成，组装，工程和移植整个细菌基因组。讨论了细菌基因组设计、组装和激活的技术进展、选择和应用。
The DNA technologies developed over the past 20 years for reading and writing the genetic code converged when the first synthetic cell was created 4 years ago. An outcome of this work has been an extraordinary set of tools for synthesizing, assembling, engineering and transplanting whole bacterial genomes. Technical progress, options and applications for bacterial genome design, assembly and activation are discussed.