Enhancing the sensitivity of colorimetric lateral flow assay (CLFA) through signal amplification techniques
Enhancing the sensitivity of colorimetric lateral flow assay (CLFA) through signal amplification techniques
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DOI：
10.1039/c8tb01603h
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发表时间：
2018-11-28
影响因子：
7
通讯作者：
Xia, Xiaohu
中科院分区：
文献类型：
--
作者：
Ye, Haihang;Xia, Xiaohu
比色侧流分析 (CLFA) 是少数可以真正走出实验室进行即时检测的诊断技术之一，无需任何设备和技术人员。尽管其简单实用，但在不增加复杂性的情况下大幅提高 CLFA 的检测灵敏度仍然是一个巨大的挑战。这种灵敏度的限制阻碍了许多关键应用，例如重要癌症和严重传染病的早期检测。近年来，随着材料科学和纳米技术的快速发展，信号放大技术有望突破CLFA现有的检测极限障碍。本文特别强调了这些用于 CLFA 开发的新兴技术。讨论了每种技术背后的基本原理以及优点和局限性。提供了对该利基和重要领域未来研究方向的展望。
Colorimetric lateral flow assay (CLFA) is one of a handful of diagnostic technologies that can be truly taken out of the laboratory for point-of-care testing without the need for any equipment and skilled personnel. Despite its simplicity and practicality, it remains a grand challenge to substantially enhance the detection sensitivity of CLFA without adding complexity. Such a limitation in sensitivity inhibits many critical applications such as early detection of significant cancers and severe infectious diseases. With the rapid development of materials science and nanotechnology, signal amplification techniques that hold great potential to break through the existing detection limit barrier of CLFA have been developed in recent years. This article specifically highlights these emerging techniques for CLFA development. The rationale behind and advantages and limitations of each technique are discussed. Perspectives on future research directions in this niche and important field are provided.