Anopheles gambiae SRPN2 facilitates midgut invasion by the malaria parasite Plasmodium berghei
Anopheles gambiae SRPN2 facilitates midgut invasion by the malaria parasite Plasmodium berghei
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DOI：
10.1038/sj.embor.7400478
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发表时间：
2005-09-01
期刊：
影响因子：
7.7
通讯作者：
Kafatos, FC
中科院分区：
文献类型：
--
作者：
Michel, K;Budd, A;Kafatos, FC
我们报告的系统发育和功能分析的基因编码的三个蚊子丝氨酸蛋白酶抑制剂（SRPN1，SRPN2和SRPN3），这类似于已知的抑制剂的酚氧化酶原激活酶在其他昆虫。在通过双链RNA注射进行RNA干扰诱导后，在冈比亚按蚊成虫中敲低SRPN 2产生了一个值得注意的表型：黑色素性假瘤的出现，其大小和数量随时间增加，表明自发的黑化和与观察到的寿命缩短相关。此外，SRPN2的敲低强烈干扰了A.冈比亚中肠被啮齿类疟疾寄生虫伯氏疟原虫感染它不影响动合子的形成，但显着减少卵囊的数量，97%，作为增加动合子溶解和黑化的结果。
We report on a phylogenetic and functional analysis of genes encoding three mosquito serpins (SRPN1, SRPN2 and SRPN3), which resemble known inhibitors of prophenoloxidase-activating enzymes in other insects. Following RNA interference induction by double-stranded RNA injection, knockdown of SRPN2 in adult Anopheles gambiae produced a notable phenotype: the appearance of melanotic pseudotumours, which increased in size and number with time, indicating spontaneous melanization and association with an observed lifespan reduction. Furthermore, knockdown of SRPN2 strongly interfered with the invasion of A. gambiae midguts by the rodent malaria parasite Plasmodium berghei. It did not affect ookinete formation, but markedly reduced oocyst numbers, by 97%, as a result of increased ookinete lysis and melanization.