Relative levels of mRNA encoding enamel proteins in enamel organ epithelia and odontoblasts
Relative levels of mRNA encoding enamel proteins in enamel organ epithelia and odontoblasts
复制标题
DOI：
10.1177/154405910308201209
复制
发表时间：
2003-12-01
影响因子：
7.6
通讯作者：
Fukae, M
中科院分区：
文献类型：
--
作者：
Nagano, T;Oida, S;Fukae, M
釉原蛋白、釉蛋白、鞘蛋白(成釉蛋白/釉蛋白)、成釉蛋白(MMP20)、KLK4(EMSP-1)是牙釉质发育过程中的主要结构蛋白和蛋白。最近，有报道成牙本质细胞表达釉原蛋白，这是最丰富的釉质蛋白。在这项研究中，我们假设成牙本质细胞表达所有的釉质蛋白和蛋白酶，并使用LightCycler仪器，通过RT-PCR检测了它们在造釉器官上皮细胞和与猪分泌期和成熟期釉质相关的成牙本质细胞中的相对mRNA水平。结果表明，分泌期EOE成牙本质细胞的成釉蛋白基因表达水平是分泌期成牙本质细胞的320倍，成熟期成牙本质细胞的成牙本质细胞的成釉蛋白mRNA水平是成熟期成牙本质细胞的2万多倍。釉质和鞘蛋白也得到了类似的结果。成牙本质细胞中成釉细胞的成釉蛋白表达水平高于分泌期EOE。这些结果支持成牙本质细胞参与牙釉质-牙本质交界处釉质层的形成。
Amelogenin, enamelin, sheathlin (ameloblastin/amelin), enamelysin (MMP-20), and KLK4 (EMSP-1) are the major structural proteins and proteinases in developing tooth enamel. Recently, odontoblasts were reported to express amelogenin, the most abundant enamel protein. In this study, we hypothesized that odontoblasts express all enamel proteins and proteases, and we measured their relative mRNA levels in enamel organ epithelia and odontoblasts associated with porcine secretory- and maturation-stage enamel by RTPCR, using a LightCycler instrument. The results showed that amelogenin mRNA in secretory-stage EOE is 320-fold higher than in odontoblasts beneath secretory-stage enamel, and over 20,000fold higher than in odontoblasts under maturation-stage enamel. Similar results were obtained for enamelin and sheathlin. Enamelysin mRNA levels were equivalent in these two tissues, while KLK4 mRNA was higher in odontoblasts than in secretory-stage EOE. These results support the conclusion that odontoblasts are involved in the formation of the enamel layer adjacent to enamel-dentin junction.