Antagonizing the Spermann organizer: Role of the homeobox gene Xvent-1
Antagonizing the Spermann organizer: Role of the homeobox gene Xvent-1
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DOI：
10.1002/j.1460-2075.1995.tb00317.x
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发表时间：
1995-12-15
期刊：
影响因子：
11.4
通讯作者：
Niehrs, C
中科院分区：
文献类型：
--
作者：
Gawantka, V;Delius, H;Niehrs, C
我们已经鉴定出一个新的同源框基因，Xvent - 1，它在非洲爪蟾原肠胚早期的腹侧边缘区差异表达。从mRNA微量注射实验中获得的证据表明，该基因在中胚层的背腹模式形成中起作用。首先，Xvent - 1由BMP - 4诱导，BMP - 4是已知的腹侧中胚层发育的关键调节因子。其次，Xvent - 1和组织者特异性基因goosecoid能够直接或间接地在一个相互调节的环路中相互作用，抑制彼此的表达，这与它们在边缘区相互排斥的表达是一致的。第三，Xvent - 1 mRNA的微量注射使背侧中胚层腹侧化。结果表明，Xvent - 1在一个维持腹侧中胚层状态并拮抗施佩曼组织者的腹侧信号通路中起作用。
We have identified a novel homeobox gene, Xvent-1, that is differentially expressed in the ventral marginal zone of the early Xenopus gastrula, Evidence is presented from mRNA microinjection experiments for a role for this gene in dorsoventral patterning of mesoderm, First, Xvent-1 is induced by BMP-4, a gene known to be a key regulator of ventral mesoderm development, Second, Xvent-1 and the organizer-specific gene goosecoid are able to interact, directly or indirectly, in a cross-regulatory loop suppressing each other's expression, consistent with their mutually exclusive expression in the marginal zone, Third, microinjection of Xvent-1 mRNA ventralizes dorsal mesoderm, The results suggest that Xvent-1 functions in a ventral signaling pathway that maintains the ventral mesodermal state and antagonizes the Spemann organizer.